Biomass production of multipopulation microalgae in open air pond for biofuel potential.

Biodiesel gains attention as it is made from renewable resources and has considerable environmental benefits. The present investigation has focused on large scale cultivation of multipopulation microalgae in open air pond using natural sea water without any additional nutritive supplements for low cost biomass production as a possible source of biofuel in large scale. Open air algal pond attained average chlorophyll concentration of 11.01 µg/L with the maximum of 43.65 µg/L as well as a higher lipid concentration of 18 % (w/w) with lipid content 9.3 mg/L on the 10th day of the culture; and maximum biomass of 0.36 g/L on the 7th day of the culture. Composition analysis of fatty acid methyl ester (FAME) was performed by gas chromatography and mass spectrometry (GCMS). Multipopulation of algal biomass had 18% of total lipid content with 55% of total saturated fatty acids (SFA), 35.3% of monounsaturated fatty acids (MUFA) and 9.7% of polyunsaturated fatty acids (PUFA), revealing a potential source of biofuel production at low cost.

Neuroprotective potential of ethanolic extract of Pseudarthria viscida (L) Wight and Arn against -amyloid(25-35)-induced amnesia in mice.

The neuroprotective potential of ethanolic extract of roots of Pseudarthria viscida (L) Wight and Arn (EEPV) was investigated against -amyloid(25-35)-induced amnesia in mice which is a suitable animal model for Alzheimer’s disease (AD). The senile plaques of -amyloid (A) are major constituents accumulated during the progression of AD as a potent neurotoxicant. In our investigation, intracerebroventricular injection of A(25-35) in mice induced the neurodegeneration, exhibited the increased time of escape latency in behavioral pattern using water maze and decreased the levels of antioxidants namley superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and vitamin C with elevated level of acetylcholinesterase enzyme (AChE). The neuroprotective potential of EEPV was determined by behavioral pattern using water maze and biochemical parameters such as SOD, CAT and GPx and vitamin C content as well as AChE. Mice were treated with EEPV at 200 and 400 mg/kg doses for 21 days. Except control, all animals received a single injection of neurotoxicant A(25-35) on 14th day. In behavioural assessment, treatment with ethanolic extract improved the cognitive function in the water maze and attenuated the elevated levels of AChE with increase in antioxidant enzymes, indicating the neuroprotection with increased levels of vitamin C. These findings suggest that ethanolic extract of P. viscida exerts anti-amnesiac effects and enhances cognitive function.

Pulmonary cavitatory tuberculosis in children.

This study was undertaken to analyse children with pulmonary cavitatory tuberculosis which is a rare and infectious condition. The pretreatment characteristics, course and response to three different anti-tuberculous regimes in 27 children with cavitary pulmonary lesions registered at the TB Clinic, Institute of Child Health, are described. Male:Female ratio was 1.2:4. Thirty per cent of affected children were below 3 years of age and had predominant lower lobe involvement whereas in older children the upper lobes were affected. Eighty five per cent of children had definite history of contact with an adult with tuberculosis. Tuberculin test was positive in 70% of children. Cavitary lesion were observed in the right lung in 66% of cases. Follow up and surveillance was carried out in 23 children who completed the anti tuberculous treatment. Regimes with isoniazid, rifampicin, pyrazinamide and streptomycin were given to different groups. Response and compliance was also monitored. Eleven out of 23 children had persistence of radiological lesions even after completion of 9 months of therapy. Inclusion of streptomycin with 2 or 3 bactericidal drugs in the intensive phase showed a better response.