ABSTRACT
The development of the Indian poultry industry in general, Namakkal, Tamil Nadu, layer industry in particular, is remarkably fast and it is growing on leaps and bounds with respect to poultry egg production. The layer birds in poultry were affected by bacterial pathogens. However, antimicrobial activities of Diplazium muricatum and Diplazium travancoricum were examined using agar diffusion method against E. coli, Bacillus and Klebsiella isolated from poultry litter collected from poultry farms. The experimental studies revealed, the crude extracts of Diplazium muricatum and D. travancoricum used in this study were inhibited the growth of microorganisms and zone of clearance ranged from 9 to 16 mm. The results indicated that D. muricatum and D. travancoricum had the potential antimicrobial compounds and great potential to use as a feed ingredient to prevent the pathogens.