ABSTRACT
In present study we investigated the anti-inflammatory effect of polyphenolic fraction isolated from Red cabbage Brassica oleracea Linn var. capitata f. rubra (PBO) on lipoplysacharide (LPS) stimulated HT-29 colonocytes and in rats with acetic acid induced colitis. Our results from in vitro studies demonstrated that PBO effectively attenuated the inflammatory response produced by cycloxygenase (COX), 5-lipoxygenase (5-LOX) and oxidative stress created via reactive oxygen species (ROS) and nitric oxide (NO) in LPS treated HT-29 cells. Additionally PBO down regulated the mRNA expression of inflammatory marker genes like COX-2, inducible nitric oxide (iNOS) tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) in HT-29 cells. PBO at a dose of 200 mg/kg body weight was tested in treatment groups of animals (n = 6) for 14 consecutive days after induction of colitis. The colonic mucosal injury was assessed by histological examination. Moreover, PBO administration markedly increased the mucin content as evidenced in periodic acid Schiff (PAS) staining and the mucosal content of lipid peroxidation (LPO), confirms that PBO could significantly inhibit colonic mucosal damage. These results indicated that PBO exert marked anti-inflammatory effect in experimental colitis, possibly by regulating the antioxidant and inflammatory mediators.
ABSTRACT
The present study evaluates the anti arthritic efficacy of triterpenoid fraction isolated from Eurphobia tirucalli Linn. (TET) in collagen induced arthritis model (CIA). TET at a dose of 100 and 200 mg /kg p.o was tested in CIA induced animals. The degree of inflammation was evaluated by hind paw swelling, arthritic index, body weight and hematological parameters. Radiological and histopathological evaluations of ankle joints were also studied. Oral administration of TET (200 mg/kg) exhibited significant (p<0.05) anti arthritic activity by decreasing the paw volume, normal body weight gain as compared to CIA groups. The altered hematological parameters like C-reactive protein (CRP) and white blood cells level in CIA groups were bring back to near normal in TET (200 mg/kg) treatment group. Further radiological and histopathological studies revealed the anti arthritic activity by indicating remodeling of ankle joints in TET treated groups. The results were parallel with standard drug diclofenac (5 mg/kg). Taken together findings from the current investigation suggest that TET has beneficial anti arthritic effect by reversing the pathological condition in a significant manner.