ABSTRACT
The present investigation is on endophytic fungus Phomopsis spp isolated from four Indian medicinal plants like., Artabotrys odoratissimus, Cassia auriculata, Guazuma ulmifolia and Terminalia catappa in four different months. Antimicrobial activity of ethyl acetate extract from the culture filtrate of Phomopsis spp were tested against six human pathogenic bacteria. Isolated three Phomopsis spp. were grown in Czapex Dox Broth for 21-days. The extracellular secondary metabolites present in the culture filtrate were extracted with ethyl acetate solvent. The extracellular bio-active compounds of the isolated fungus were tested for its anti microbial potential in well diffusion method, against three, Gram-positive and Gram-negative bacteria such as Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Escherichia coli, Klebsiella Pneumoniae and Pseudomonas aeruginosa. Among all the three Phomopsis spp, the extract obtained from Phomopsis sp.2 exhibited a promising activity against the entire test bacteria. This bioactivity compounds focus on clinical pharmacology to identify a novel therapeutic targets and it can be easily scaled up for the large-scale commercial production.