ABSTRACT
Compared with acromioclavicular dislocation, dislocation of the clavicle at its sternal end is uncommon and accounts for 3% of all injuries to the shoulder girdle. Furthermore, the posterior dislocation of the sternoclavicular joint is relatively a rare injury compared to the other types of sternoclavicular dislocation. We report this case since we have experience with similar cases of traumatic posterior dislocation at the sternoclavicular joint, which were successfully treated with x-ray guided reduction.
Subject(s)
Clavicle , Joint Dislocations , Shoulder , Sternoclavicular JointABSTRACT
Bisphosphonate is notable for the treatment of osteoporosis in the world. But recently if Bisphosphonate is taken for a long time, it causes an insufficiency fracture by suppression of bone turn-over and it is reported rarely on femur neck. Here we report a case of insufficiency fracture on ipsilateral femur neck in woman treated with long term Bisphosphonate therapy.
Subject(s)
Female , Humans , Femur , Femur Neck , Fractures, Stress , OsteoporosisABSTRACT
PURPOSE: To evaluate clinical results after anterior plate fixation for humeral shaft fractures. MATERIALS AND METHODS: We evaluated 20 patients with a humeral shaft fracture. Anterior plate fixation with anterolateral approach to the humeral shaft was performed in all cases. Time to fracture healing, presence of complications such as radial nerve injury, and range of motion of the affected shoulder and elbow joint were assessed. RESULTS: All fractures were united and the mean healing time was 16 weeks (range, 12 week to 1 year). There were one nonunion and one postoperative radial nerve palsy. The nonuion was treated with a longer plate and bone grafting, resulting in union. The radial nerve palsy recovered at 4 months after operation. Two preoperative radial nerve injury recovered spontaneously at 6 to 8 months. The mean flexion of elbow was 125degrees and mean extension was 0degrees. The mean flexion of shoulder was 170degrees and mean extension was 170degrees. CONCLUSION: Internal fixation of humeral fractures using anterior plating technique is recommended as a reliable method to regain satisfactory range of motion of the elbow and shoulder.
Subject(s)
Humans , Bone Transplantation , Elbow , Elbow Joint , Fracture Healing , Humeral Fractures , Paralysis , Radial Nerve , Range of Motion, Articular , ShoulderABSTRACT
Tardy ulnar nerve palsy might develop secondary to nonunion, malunion, or elbow deformity after medial epicondylar fracture of the humerus. We report a case of tardy ulnar nerve palsy following medial epicondylar fracture, treated with excision of bony fragment, neurolysis and relocation of the ulnar nerve.
Subject(s)
Congenital Abnormalities , Joint Dislocations , Elbow , Humerus , Ulnar Nerve , Ulnar NeuropathiesABSTRACT
We have now construted a novel recombinant baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) producing polyhedra with Bacillus thuringiensis (Bt) Cry1Ac crystal protein. The recombinant polyhedra produced by the recombinant baculovirus, Btrus, in insect cells was characterized. The recombinant baculovirus has two independent transcription units in opposite orientations with two promoters, p10 or polyhedrin gene promoter each initiating transcription of either native polyhedrin or fusion protein with polyhedrin and Bt Cry1Ac crystal protein. Suprisingly, this recombinant baculovirus stably produced recombinant polyhedra which were nearly similar to those of wild-type AcNPV. The immunogold staining experiment showed that the recombinant polyhedra were assembled with polyhedrin and Bt Cry1Ac crystal protein, and contained virus paticles. Insecticidal toxicity of recombinant polyhedra of Btrus to the fall webworm, Hyphantrea cunea, was strikingly improved in comparison with the wild-type AcNPV.
Subject(s)
Bacillus thuringiensis , Bacillus , Baculoviridae , Insecta , NucleopolyhedrovirusesABSTRACT
We have expressed GFP in Sf9 and Bm5 cells or Bombyx by larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of Ac-Bm hybrid virus expressing P-galactosidase was cotransfected with baculovirus transfer vector containing GFP gene, pBacPAK-GFP in Sf9 cells. The Ac-Bm hybrid virus harboring GFP was named as Ac-Bm hybrid virus-GFP. The Ac-Bm hybrid virus-GFP-infected insect cells were easily selected by detecting the emission of GFP from each well of cell culture dish on the UV illuminator. GFP produced by Ac-Bm hybrid virus-GFP in Sf9 and Bm5 cells or B. mori larvae was confirmed by SDS-PAGE and Western blot analysis using GFP antibody. In addition, B. mori larvae infected with Ac-Bm hybrid virus-GFP was apparently appeared fluorescence from the whole body at 5 days postinoculation. The fluorescence of GFP from the hemolymph and fat body of B. mori larvae infected with Ac-Bm hybrid virus-GFP was also observed by fluorescence microscope. In conclusion, our results demonstrated that in baculovirus expression vector system, use of Ac-Bm hybrid virus have an additional advantage of expanded host range for producing recombinant proteins.
Subject(s)
Animals , Baculoviridae , Blotting, Western , Bombyx , Cell Culture Techniques , DNA , Electrophoresis, Polyacrylamide Gel , Fat Body , Fluorescence , Hemolymph , Host Specificity , Insecta , Larva , Recombinant Proteins , Sf9 Cells , SpodopteraABSTRACT
To use recombinant viruses with wider host range as viral insecticides, we investigated the characteristics and pathogenicity of host range expanded recombinant viruses in insect cells. We compared host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Autographa californica nuclear polyhedrosis virus (AcNPV) and Bombyx mori NPV (BmNPV), to host range expanded AcNPV, Ac-BH, by substitution of the 0.6 Kb fragment of the BmNPV helicase gene. Restriction endonuclease profiles of RecS-B6 and RecB-8 DNAs were different from those of parent viruses. Nucleotide sequence analysis of the 0.6 Kb region in the putative helicase gene of RecS-B6 and RecB-8 showed that their structures were identical to the counterpart region of BmNPV Comparison of viral replication of these recombinant viruses in Sf-21 and BmN-4 cells showed that Ac-BH, compared to wild type viruses, replicated well in BmN-4 cells but poorly in Sf-21 cells. In contrast, RecS-B6 and RecB-8 replicated relatively well in both cells compared to parent viruses. These results may imply that random genomic recombinant viruses, RecS-B6 and RecB-8, possess better potential as viral pesticides than helicase-mediated recombinant virus, Ac-BH.