ABSTRACT
We were studied on differentiation potential of CD133[+] and CD34[+] hematopoietic stem cells into motor neuron like cells
Materials and methods: CD133[+] and CD34[+] HSCs were isolated from human UCB using MACS system. After cell characterization using flow cytometry, the cells were treated with a combination of Retinoic acid, Sonic hedgehog, Brain derived neurotrophic factor, and B27 through a 2- step procedure for two weeks. The expression of MN-specific markers was examined using immunocytochemistry and flow cytometery
Results: Flow cytometery analysis revealed 98 percent and 95.7 percent CD133[+] and CD34[+] cells, respectively. By the end of the two-week differentiation protocol, CD133[+] and CD34[+] cells acquired unipolar MNL morphology with thin and long neurites. The expression of Isl-1, AChE, NF-H and Nestin was detected in 66.4 percent, 58.3 percent, 80.6 percentand 84.9 percent of CD133[+] cells and 63.2 percent, 52.3 percent, 78.6 percent and 80.1 percent of CD34[+] cells
Conclusion: Human UCB - CD133[+] and CD34+HSCs are remarkably potent cell candidates to trans- differentiate into motor neuron-like cells, in vitro