ABSTRACT
Background: ATP-binding cassette transporter A1 [ABCA1] is a membrane integral protein which plays a vital role in High Density Lipoprotein [HDL] metabolism and exerts a protective effect against Hypoalphalipoproteinemia [HA] by mediation of rate-limiting step in HDL biogenesis. In addition, this protein possesses anti-inflammatory effects by inhibiting the production of some inflammatory cytokines in macrophages. This study investigated the association of ABCA1-565 C/T gene polymorphism with HA and serum lipids, IL-6 and CRP levels
Methods: A population which consisted of 101 HA and 95 normal subjects were genotyped for ABCA1-565C/T polymorphism by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism [PCR-RFLP]. The serum concentrations of lipids, IL-6 and high sensitive-CRP [hs-CRP] were measured by the relevant methods
Results: The frequency of T allele was significantly higher in the HA group than the controls [31.7 vs. 19.5%, p=0.002]. Thus, carriers of the T allele [CT and TT genotypes] had a higher risk for HA [p=0.016, OR=2.04, 95% CI=1.14-3.63]. T allele carriers demonstrated decreased HDL-C and increased triglyceride, IL-6 and CRP levels than those with the CC genotype
Conclusion: This study suggests that the-565 C/T polymorphism of ABCA1 gene is associated with an increased risk of HA, decreased HDL-C and increased TG, IL-6 and CRP