ABSTRACT
Dorema aucheri is a plant that grows in Iran. In Persian it is called [Bilhar]. This experimental study was carried out at Ferdowsi University of Mashhad in 2014. After collection and preparation of aqueous and ethanolic extracts of Dorema aucheri [Bilhar], The antibacterial activity of ethanolic and aqueous extracts of Bilhar was evaluated against 7 laboratory strains of microorganisms, including 4 Gram positive [Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus and Bacillus subtilis] and 3 Gram negative bacteria [Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris]. Its effects against human pathogen microorganism were determined using "Spreading of the Extract on Medium Surface" and "Disk Agar Diffusion Method", Minimal Inhibitory Concentration [MIC] and Minimum Lethal Concentration [MLC] were determined for this extract. Collected data were analyzed by SPSS software using one-way ANOVA. The zone of inhibition for the ethanolic extract varied from 8 mm for P. aeruginosa to 24 mm for S. pyogenes and from 7 mm for P. aeruginosa to 19 mm for S.pyogenes in the aqueous extract. The minimum inhibitory concentration [MIC] of the extracts ranged between 2 mg/ml and 64 mg/ml while the minimum lethal concentration [MLC] ranged between 4 mg/ml and 256 mg/ml. Among of tested strains, P. aeruginosa has maximum MIC and MBC. 30 and 40 mg/mL Concentrations of Redcurrant have significant antimicrobial effect on bacteria. Antibacterial effect of extracts was decreased with decrease of extract concentration in disk. According to result, ethanolic extract of Dorema aucheri have antimicrobial effect on growth of all of the strains exposed analyzes and antimicrobial effect of that was maximum on Gram-positive bacterum of S. pyogenes. P. aeruginosa showed the highest level of resistance against the aqueous and ethanolic Bilhar extracts. The present study demonstrated that the ethanol leaf extract of Dorema aucheri hold an excellent potential as an antibacterial agent
ABSTRACT
The aim of this paper was to investigate the antibacterial potential of Kelussia odoratissima Mozff extract against Gram-negative and Gram-positive bacteria. Karafs-eKoohi with the scientific name of Kelussia odoratissima is an Iranian endemic edible plant in the middle region of Iran with enormous use as food, spice and medicinal herb. The antibacterial effect of the extracts was investigated using pour plate and disk diffusion methods. Minimum Inhibitory Concentration [MIC] and Minimum Bactericidal Concentration [MBC] were also studied using the dilution method. Repeated measure ANOVA was used for data analysis. The results showed that in disk diffusion method all concentrations of ethanolic extract had inhibitory effect against Bacillus subtilis and Staphylococcus aureus. Minimum Inhibitory Concentration [MIC] of Kelussia odoratissima leaves of aqueous and ethanolic extracts for Bacillus subtilis and Staphylococcus aureus were 16 and 8 mg/ml, and for Enterobacter aerogenes were 32 and 16 mg/ml, respectively. Minimum Bactericidal Concentration [MBC] of Kelussia odoratissima leaves of aqueous and ethanolic extracts for Bacillus subtilis and Staphylococcus aureus were 32 and 16 mg/ml, and for Enterobacter aerogenes were 64 and 32mg/ml, respectively. The results showed that the extract of Kelussia odoratissima had a satisfactory antimicrobial activity and the ethanolic extract of Kelussia odoratissima leaves had greater inhibitory effects on the strains studied compared to aqueous extract in vitro. A significant correlation was also observed between zone of inhibition and concentration of extracts