Significant correlation of angiotensin converting enzyme and glycoprotein IIIa genes polymorphisms with unexplained recurrent pregnancy loss in north of Iran

Background: Spontaneous abortion is considered as the most complex problem during pregnancy. Thrombophilia is resumed as a cause of recurrent pregnancy loss [RPL]. Glycoprotein IIIa [GPIIIa] gene is involved in thrombosis and abortion. Angiotensin converting enzyme [ACE] converts angiotensin I to angiotensin II and is involved in thrombosis. The most common polymorphism in this gene is the insertion/deletion [I/D]

Objective: In this study, we analyzed the association between ACE I/D and GPIIIa c.98C >T polymorphisms in women with unexplained RPL from the north of Iran

Materials and Methods: Sample population consisted of 100 women with unexplained RPL and 100 controls. The ACE I/D and GPIIIa c.98C>T polymorphisms were genotyped by TETRA-ARMS PCR. The association between genotypes frequency and RPL were analyzed using ?P2P and exact fisher tests. Associated risk with double genotype combinations was also investigated by binary logistic regression

Results: There was significant association between ACE DD genotype and RPL [OR=2.04; 95% CI=0.94-4.44; p=0.036]. ACE D Allele was also significantly associated with the RPL [OR=1.59; 95% CI=1.05-2.41; p=0.013]. No significant association was observed between GPIIIa c.98C>T polymorphism and RPL

Conclusion: ACE I/D polymorphism may probably be a prognostic factor in female family members of women with the history of recurrent abortion

Frequencies of two CYP2C19 defective alleles [CYP2C19[asterisk]2, and [asterisk]3] among Iranian population in Mazandaran Province

Cytochrome P450 2C19 [CYP2C19] is a polymorphically expressed enzyme that shows marked interindividual and interethnic variation. CYP2C19[asterisk]2 and CYP2C19[asterisk]3 are the most frequent identified defective alleles in Orientals and Caucasian poor metabolizers [PM]. The aim of this study was to investigate the frequencies of CYP2C19[asterisk]1, CYP2C19[asterisk]2 and CYP2C19[asterisk]3 alleles and CYP2C19 genotypes among Mazandarani ethnic group among Iranian Population. The study was conducted on 103 unrelated healthy volunteers. DNA was extracted from leucocytes and analyzed by the PCR-RFLP protocol. The PCR product was digested with restriction enzymes [SmaI and BamH1] and then separated electrophoretically using polyacrylamide gel. Of the tested alleles, CYP2C19[asterisk]1, and CYP2C19[asterisk]2, but not CYP2C19[asterisk]3, were detected. The frequencies for CYP2C19 alleles [asterisk]1, [asterisk]2, and [asterisk]3 were 91%, 9.0%, and 0.0%, respectively. CYP2C19 genotypes [asterisk]1/[asterisk]1, [asterisk]1/[asterisk]2, [asterisk]1/[asterisk]3, [asterisk]2/[asterisk]2, [asterisk]2/[asterisk]3 and [asterisk]3/[asterisk]3 frequencies were 84%, 14%, 0.0%, 2.0%, and 0.0%, respectively. The result of the present study shows that the two inactive alleles of CYP2C19 accounted for 9.0% of CYP2C19 alleles in our sample versus 8.8 - 40.1% reported in other populations. The frequency of alleles was significant. Differences between our sample and African and Eastern Asian populations