ABSTRACT
Background: Citrullus colocynthis [CCT] is used as the anti-diabetic and antioxidant agent. Polycystic ovarian syndrome [PCOS] is a reproductive disorder which level of gonadotropins and sexual hormones are imbalanced
Objective: We evaluated the effect of CCT hydro-alcoholic extract on hormonal and folliculogenesis process in estradiol valerate-induced PCOs rats' model
Materials and Methods: 40 female adult Wistar rats divided into five groups [n=8 each: Group I [control] only injected by sesame oil as estradiol valerate solvent, group II [Sham] was orally received normal saline after estradiol valerate- induced polycystic ovarian syndrome [4 mg/rat estradiol valerate, intramuscularly], and three experimental groups, that after induction of PCOS within 60 days, received orally 50 mg/kg CCT extract [group III], 50mg/kg metformin [group IV], and CCT extract+ metformin [group V] for 20 days. The serum concentration level of luteinizing, testosterone and follicle stimulating hormones were measured using ELISA method and the serum concentration level of glucose were measured using the oxidative method [glucose meter]. Histological study of ovary tissue carried out by hematoxylin-eosin staining
Results: There was a significant reduction in luteinizing hormone and testosterone in III-V groups compared to Sham group, whereas follicle stimulating hormone in III-V groups was not significantly changed in comparison with Sham group. Histological investigations showed a significant increase in number of preantral and antral follicles and corpus luteum in the experimental groups compared to group II
Conclusion: Marked improvement in hormonal and histological symptoms of PCOS may be due to CCT effects hence, CCT can potentially be considered as an effective drug for treatment of PCOS
ABSTRACT
Background: meiotic genes are very important candidates for genes contributing to female and male infertility. Mammalian MutL homologues have dual roles in DNA mismatch repair [MMR] after replication errors and meiotic reciprocal recombination. The MutL homologs, MLH1 and MLH3, are crucial for meiotic reciprocal recombination and human fertility. In this study the functional polymorphisms of MLH3C2531T was investigated in Iranian women with unexplained infertility
Objective: investigating the association between a common SNP [single nucleotide polymorphism] C2531T in the MLH3 gene and female infertility
Materials and Methods: in total, 105 women with unexplained infertility as case group and 100 women with at least one child and no history of infertility or abortion as controls were recruited for this association study. The MLH3 C2531T polymorphism was tested by tetra-amplification refractory mutation system-PCR [4P-ARMS-PCR] method
Results: the MLH3 2531C and T alleles frequencies were 43.33% and 56.67% among infertile patients, and 61.5% and 38.5% among normal controls, respectively. In the patient and control subjects the CC [Pro 844 Pro] genotype frequency ofMLH3 C2531T was 4.76% and 25%, the CT [Pro 844 Leu] genotype was 77.15% and 73%, and the TT [Leu 844 Leu] genotype was 19% and 2%, respectively [p=0.0001]
Conclusion: the presence of the polymorphic allele T leads to an increased risk of 2.09 times [OR=2.09, 95% CI=1.38-3.16; p=0.0001] for developing infertility in relation to the control group. Therefore, our data suggest that the MLH3 C2531T polymorphism can be associated with the risk of unexplained infertility in Iranian women