ABSTRACT
Background: methamphetamine [MA] was shown to have harmful effects on male reproductive system
Objective: to investigate probable effects of daily administration of MA on sperm parameters and chromatin/DNA integrity in mouse
Material and Methods: thirty-five NMRI male mice were divided into five groups including low, medium, and high dosage groups which were injected intraperitoneally with 4, 8 and 15 mg/kg/day for 35 days, respectively. Normal saline was injected in sham group and no medications were used in control group. Then, the mice were killed and caudal epididymis of each animal was cut and placed in Ham's F10 medium for sperm retrieval. To evaluate sperm chromatin abnormalities, the aniline blue, toluidine blue and chromomycine A3 were used. For sperm DNA integrity and apoptosis, the acridine orange, sperm chromatin dispersion, and TUNEL assay were applied. For sperm morphology, Papanicolaou staining was done
Results: normal morphology and progressive motility of spermatozoa decreased in medium and high dosage groups in comparison with the control group [p=0.035]. There was a significant increase in rate of aniline blue, toluidine blue, and chromomycine A3 positive spermatozoa in high dosage group. In a similar manner, there was an increase in rates of acridine orange, TUNEL and sperm chromatin dispersion positive sperm cells in high dosage group with respect to others
Conclusion: MA abuse in a dose-dependent manner could have detrimental effects on male reproductive indices including sperm parameters and sperm chromatin/DNA integrity in mice
ABSTRACT
Background: To meet the future challenges in the field of reproductive medicine in Iran, better understanding of published studies is needed. Bibliometric methods and social network analysis have been used to measure the scope and illustrate scientific output of researchers in this field
Objective: This study provides insight into the structure of the network of Iranian papers published in the field of reproductive medicine through 2010-2014
Materials and Methods: In this cross-sectional study, all relevant scientific publications were retrieved from Scopus database and were analyzed according to document type, journal of publication, hot topics, authors and institutions. The results were mapped and clustered by VosViewer software
Results: In total, 3141 papers from Iranian researchers were identified in Scopus database between 2010-2014. The numbers of publications per year have been 12Tincrease12Td from 461 in 2010 to 749 in 2014. Tehran University of Medical Sciences and [Soleimani M] are occupied the top position based on Productivity indicator. Likewise [Soleimani M] was obtained the first rank among authors according to degree centrality, betweenness centrality and collaboration criteria. In addition, among institutions, Iranian Academic Center for Education, Culture and Research [ACECR] was leader based on degree centrality, betweenness centrality and collaboration indicators
Conclusion: Publications of Iranian researchers in the field of reproductive medicine showed steadily growth during 2010-2014. It seems that in addition to quantity, Iranian authors have to promote quality of articles and collaboration. It will help them to advance their efforts
ABSTRACT
About 15% of couples have infertility problems which 40% of them are related to the male factors. Genetic factors are candidate for about 10% of male infertility conditions. Among these, AZFa, AZFb, AZFc and AZFd regions on the Yq are considered most important for spermatogenesis. Microdeletions of these regions are thought to be involved in some cases of azoospermic or oligospermic infertile men. We studied the prevalence of AZF microdeletions among Iranian infertile men with non-obstructive azoospermia and oligospermia. A total of 50 Iranian azoospermic and oligospermic infertile men were selected for case group and 50 men with normal spermogram as control group. The molecular study of Y chromosome microdeletions was done by multiplex polymerase chain reaction [M-PCR] method by using of 13 sequence tagged site [STS] markers from AZF region. Four [8%] patients showed Y chromosome microdeletions among case group, deletion in AZFc region was the most frequent [80%] followed by AZFb [20%], in AZFa and AZFd region we did not detect any deletions. No deletion was detected in control group; the ratio of Y chromosome microdeletion in azoospermic men was higher than this ratio in oligospermic men [19% [3/16] among azoospermic men and 3% [1/34] among oligospermics]. Serum FSH level in men with microdeletions was higher than this level in men with no deletions [p=0.034]. Because of relatively high prevalence of microdeletions on the long arm of Y chromosome among Iranian azoospermic and oligospermic patients, screening of this microdeletion may be advised to infertile men particularly azoospermic and oligospermic men before using assisted reproductive treatments