ABSTRACT
Background: Black Seed [BS] is used in traditional medicine as a therapy for a variety of diseases including allergic asthma
Materials and Methods: In the present study, anti-inflammatory and immunomodulatory effects of BS on cytokine gene expression, lung airway eosinophilia and goblet cell hyperplasia were examined in a mouse mice model of allergic asthma. Groups of 6-week-old female BALB/c mice were sensitized by intraperitoneal injections of OVA plus alum on days 1 and 14. On days 24, 26, 28 and 30, the mice were exposed to OVA in saline for 30 min with nebulizer. Similar experiments were conducted with mice receiving saline as a negative control
Results: The mouse allergic asthma model received BS by food on days 23, 25, 27 and 29. Then, the percentage of inflammatory cells as well as mRNA expression levels of interleukin [IL]-4, IL-5, IL-13 and mucin [MUC5a] genes were survived in Broncho-alveolar lavage fluid [BALF]. Furthermore, we attempted to examine histopathological examination of the lung. Mice receiving BS showed a significant decrease in the number of eosinophils, and a potential inhibitory effect on mRNA expression levels of Th2-driven immune response cytokines and mucin, resulting in decreased production of interleukin and mucin in allergic asthma
Conclusion: Our findings suggested that BS has an anti-inflammatory and immunomodulatory effect during the allergic response in the lung, and can be a promising treatment for allergic asthma in humans
ABSTRACT
Background and Aims: aspergillus fumigatus is a sporadic fungus that causes different infections and allergies in immunocompromised patients. The allergic disease caused by this fungus is called allergic bronchopulmonary aspergillosis [ABPA]. ABPA is considered important in atopic and immunocompromised individuals, which can result in inflammation and epithelial damage. Therefore, the aim of this study was to evaluate the T helper [Th] 2 responses in a ABPA murine model by measuring the main cytokines involved in Th2
Materials and Methods: twenty male BALB/c mice were divided into two groups of 10 mice each: control and ABPA group. ABPA was induced by inhalation of A. fumigatus conidia intranasally. Total and specific IgE were measured in the mice sera. Levels of cytokines in broncho alveolar lavage [BAL] of under studied groups were measured by Enzyme-linked immunosorbent assay three weeks after the treatment
Results: the obtained results indicated that total and specific IgE increased in the ABPA group [p<0.05]. The levels of Interleukin [IL]-4, IL-5 and IL-13 in brocho alveolar lavage of ABPA group was significantly higher than the control group [p<0.05], whereas interferon-gamma levels did not reveal any significant differences between the studied groups
Conclusions: the findings of the present study confirmed the role of Th2 cytokines in the ABPA reactions. However, more comprehensive studies are necessitated to determine the exact mechanisms of immune responses to ABPA as well as the role of Th1/Th2 responses in control of ABPA reactions. Regulation of Th2 responses could nbe regarded as a potential therapy for ABPA as well
ABSTRACT
HBV is a non-cytopathic virus and cell mediated immune response against this. Humoral mediated immune response are responsible for allergic diseases. Balance between these two subsets of Th CD4+ cells are result of the immune system response. A 56 year old woman presented with chronic HBV infection, allergic asthma, type 2 diabetes mellitus and high blood pressure and high blood lipid. Patients should be followed for the allergic and autoimmune diseases along with their viral reactivation.