ABSTRACT
Leptospirosis is a worldwide zoonosis caused by Leptospira interrogans. Leptospirosis results in decreased milk production, abortion, stillbirth, infertility and mortality, which causes financial loss in the cattle industry. The aim of this research was to perform a serological and bacteriological study of leptospirosis in 6 industrial dairy herds and 3 feedlots with previous records of leptospirosis in Tehran suburbs in 2011-2012. For the purpose of this study, 408 blood samples from dairy cattle and 154 blood samples from feedlots were collected using sterile 10ml venoject vacutainers from tail vein. Two months later, 118 urine samples were collected from 20% of the two groups of serological negative and positive animals. All serum samples were serologically tested by microscopic agglutination test [MAT], a standard method for serological diagnosis of leptospirosis. The serum samples were tested for antibodies against five live antigens of Leptospira interrogans serovars: Pomona, Grippotyphosa, Hardjo, Icterohaemorrhagiae and Canicola. Urine samples were used for bacteriological isolation of Leptospira spp. Serological results showed that 228 [40.6%] of animals had a positive reaction against one or more serovars. The most prevalent Leptospira serovars was Pomona 118 [40.3%] and the least prevalent was Canicola 4 [1.4%]. The most prevalent titer was 1:100, and the highest titer was 1:1600. Also the most seropositive cases were observed in 3 to 4-year-old cows. Bacteriological results revealed that in 11 [9.3%] urine samples Leptospira spp. were isolated, all taken from one feedlot farm. According to the history taken from each farm, the main risk factors were the presence of rodents and low hygienic conditions of the farms. The results of this study revealed that cows could have a major role in maintaining Pomona, Grippotyphosa and Hardjo serovars; indeed, they are a potential zoonotic risk to slaughter house workers, meat inspectors, milkers and farmers
ABSTRACT
The present study was done from winter 2003 until spring 2004. Faecal Samples[n = 400] were collected from calves and cattle in four age groups:0-6,7-12,13-24 months and 2-10 years old.Sample were stained by modified Ziel-Nelson method and oocytes of cryptosporidium were diagnosed on the basis of their sporozoiets. Infection rate was equal amomg animals during all seasons. Adult cattle had the highest infection rate in the healthy ones [23.75%]. on the other hand, the highest prevalence rate of infection in the diarrheic animals was seen in the calves. While bovine cryptosporidiosis accompanying is with diarrhea in the < 6 months of age, it has not any clinical signs in the adult ones.The present animals in Khorram-Abad[17.5%] were infected