ABSTRACT
The aim of this study was to examine the possible morphological and morphometric changes resulting from vitrification of embryos at the cleavage stage. In this study, 30 mice early-cleavage embryos at different stages of cleavage, resulting from in vitro fertilization [IVF] techniques, were examined before and after vitrification. Digital images were taken from embryos before and after vitrification. Zona pellucida thickness, differences in zona pellucida thickness, and diameter and volume of blastomeres and embryos as morphometric parameters and current rating of appearance of embryos as morphological parameters, have been studied. According to our findings, there were significant mean differences in all morphometric parameters of the two groups except in the zona pellucid thickness [P
ABSTRACT
Management of cutaneous wounds is an important subject in medicine. Different chemical agents have been used for wound healing but each of them has side effects. In this study, we examined the effects of an experimental ointment derived from a natural product on wound healing. Twenty-four white rabbits divided into three groups as follows: control, Eucerin and Gazangebine. The ointment was made by Gazangebine extract [9%] and Eucerin in the laboratory. A full thickness wound [2cmx2cm] was made on the left flank of the test animals. The ointment was applied to the wound area twice daily without any covering as an open wound. Wound area was measured every 3 days until day 18. In this day, rabbits were biopsied to look for histopathological changes. Gazangebine ointment had no beneficial effects on wound contraction. However, it decreased inflammation and neutrophil number [P<0.05, p=0.001] and increased eosinophil number and dermal tissue healing [p<0.05, p=0.000]. The results suggest that Gazangebine ointment has positive effects on wound healing
Subject(s)
Animals , Rabbits , Ointments , LipidsABSTRACT
Cisplatin is a potent antineoplasic drug. The beneficial effects of the drug are limited by its nephrotoxicity. The purpose of this study IS to Introduce stereological methods [Estimation of mean glomerular volume] to determine the toxicity of drug and examine the Gahum apanne [a traditional herbal plant] as a protective agent. This experimental study was done in histology department in Ahwaz University of medical sciences in 1380. 70 Spraque-Dawally male rats Were iselected randomly and divided into 7 groups as below: two control groups [one for drug and one for plant], five experimental groups; one treated by acute dose of drug [7.5 mg/kg, IP] and other groups treated with 4 doses of hydroalcholic extract of plants [4, 8, 16, 32 mg/kg] half an hour before receiving drug orally. Animals were scarified 96 hour after receiving the drugs and their right kidneys were processed for stereological assessment based on cavalier method. Statistical analysis of data from calculation of mean glomerular volume was done by ANOVA and two sided-dunet test, using SPSS software. The results based on cavalier method, showed an increase in mean glomerular volume in all the groups that were treated with drug, whereas lower grade of glomerulomegaly was seen in those groups which received higher doses [16 and 32 mg/kg] of plant's extract [p<0.05]. Nephrotoxicity induced by cisplatin was even observed in quantitative parameters of kidney [e.g. increasing the mean glomerular volume] but by using Galim apain's extract this side effect can be reduced significantly. Moreover stereological techniques are valuable tools for studying the drug effects in all organs of the body
ABSTRACT
The use of stereological methods in evaluation of volume changes in vital organs such as kidney is very important. In this study, the volume of glomeruli in glycerol-induced acute renal failure in rats was determined. 48 Sprague-Dawley male rats were divided into 2 groups [1control, 1experiment]. Experimental group was injected 10ml/kg of 50% glycerol intramuscularly, while the same volume of saline was injected in the control group. After 48h, animals were dissected under deep anesthesia, and right kidneys were fixed by vascular perfusion. Microscopic slides were stained with H-E. Glomerular volumes were calculated using projecting microscope, point counting and cavalieri principal. The results indicated that renal failure induced-glycerol didn't affect the glomerular volume. It seems that estimation of glomerular volume can not be a useful variable in identification of acute renal failure induced glycerol, but further investigations are needed to clarify the issue