ABSTRACT
Objective: To determine the effects of lead acetate and Ficus carica on disruption of basement membrane in seminiferous tubules of adult rat testis. Study Design: An experimental study. Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi in collaboration with National Institute of Health [NIH], Islamabad, from March to November 2017
Methodology: Thirty male adult Sprague Dawley rats were selected and divided into three groups, each with ten animals. All treatments were given once daily for a period of eight weeks. Control was labelled as group A. Group B was administered lead acetate at a dose of 30 mg/kg body weight. Group C was treated with lead acetate at a dose of 30 mg/kg body weight and Ficus carica at a dose of 80 mg/kg body weight. Animals were dissected 24 hours after the last dose. Testis were treated, fixed and stained for histological study. Disruption of basement membrane in seminiferous tubules was scored morphometrically on a scale of 0 [normal] to 3 [>70% tubules showing disruption] and statistically analysed
Results: Significant number of seminiferous tubules showed disruption of basement membrane in group B [20%] as compared to group A [0%]. Less severe disruption of membrane was seen in group C as compared to group B, which was statistically not significant [p=0.082]
Conclusion: Lead acetate causes significant disruption of basement membrane in seminiferous tubules of testis of adult rats but subsequent administration of Ficus carica reduces the effects on short term
ABSTRACT
Objective: To determine the effects of lead acetate and Ficus carica on disruption of basement membrane in seminiferous tubules of adult rat testis
Study Design: An experimental study
Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi in collaboration with National Institute of Health [NIH], Islamabad, from March to November 2017
Methodology: Thirty male adult Sprague Dawley rats were selected and divided into three groups, each with ten animals. All treatments were given once daily for a period of eight weeks. Control was labelled as group A. Group B was administered lead acetate at a dose of 30 mg/kg body weight. Group C was treated with lead acetate at a dose of 30 mg/kg body weight and Ficus carica at a dose of 80 mg/kg body weight. Animals were dissected 24 hours after the last dose. Testis were treated, fixed and stained for histological study. Disruption of basement membrane in seminiferous tubules was scored morphometrically on a scale of 0 [normal] to 3 [>70% tubules showing disruption] and statistically analysed
Results: Significant number of seminiferous tubules showed disruption of basement membrane in group B [20%] as compared to group A [0%]. Less severe disruption of membrane was seen in group C as compared to group B, which was statistically not significant [p=0.082]
Conclusion: Lead acetate causes significant disruption of basement membrane in seminiferous tubules of testis of adult rats but subsequent administration of Ficus carica reduces the effects on short term
ABSTRACT
Objective: To study and compare the histomorphological changes induced by second generation [2G], third generation [3G] and fourth generation [4G] mobile phone electromagnetic fields on the organization of Purkinje cell layer of rat cerebellum
Study Design: Laboratory based randomized control trial
Place and Duration of Study: The study was carried out in the Anatomy department, Army Medical College Rawalpindi; in collaboration with animal house, National Institute of Health, Islamabad, from Nov 2014 to Nov 2015
Material and Methods: Forty adult Sprague Dawley rats [20 male, 20 female], weighing 250-350grams,were taken and divided into 4 groups with 10 rats [5 male, 5 female] in each group. Group A served as control and was given normal diet and water ad libitum. Groups B, C and D were exposed to EMF from 2G, 3G and 4G mobile phones respectively, daily for 1 hour for 2 months. The animals were sacrificed on 60th day of the experiment, cerebellums were removed, fixed in 10% formalin, processed and stained with haematoxylin and eosin [H and E] for histological study
Results: It was observed on microscopic examination that EMF from 2G, 3G and 4G mobile phones effected the organization of Purkinje cell layer of cerebellum; being double cell layer in group B and multiple cell layers in groups C and D
Conclusion: It was concluded from current results that radiations from 2G, 3G and 4G mobile phones have deleterious effects on the organization of Purkinje cell layer of cerebellum with 3G and 4G causing more harm as compared to EMF from 2G mobile phones
Subject(s)
Animals, Laboratory , Female , Male , Adult , Purkinje Cells/physiology , Cerebellum , Rats, Sprague-Dawley , Randomized Controlled Trials as TopicABSTRACT
Objective: To study the protective role of omega 3 fatty acids [omg 3 FAs] on the histomorphological changes in the height of the prostatic epithelium in rats induced by sleep deprivation
Study Design: Lab based randomized control trial
Place and Duration of Study: The study was conducted at Anatomy Department, Army Medical College, Rawalpindi, in collaboration with National Institute of Health [NIH], Rawalpindi for duration of one year, from Nov 2014 to Nov 2015
Material and Methods: Thirty male Sprague Dawley rats, 3-4 months of age with average weights of 200-300 grams [gm] were divided in three groups each having 10 rats. Group A served as control with standard lab diet and regular sleep -wake cycle. Group B was subjected to sleep deprivation of 16 hours followed by a sleep window of 8 hrs daily for 2 months and group C was administrated with omg 3 fatty acids [FAs] and was sleep deprived as group B for 2 months. At the end of the experimental period rats were anesthetized and their blood sample was drawn for hormonal assay. They were dissected and the prostate gland was removed and fixed in 10 percent formalin. Five micrometer [micro m] sections were obtained after tissue processing and stained with haematoxylin and eosin [HandE] for histological study
Results: Microscopic examination revealed that the epithelium of glandular acini was columnar in group A. Marked decrease in the height of cells was observed in group B whereas the epithelium was nearly cuboidal in group C
Conclusion: It was concluded that sleep deprivation had deleterious effects on the epithelium of the prostatic acini and that Omega 3 fatty acids had a protective effect on the epithelium of the prostatic acini
ABSTRACT
Objective: To determine the effect of soyabean oil supplementation on perivascular inflammation in lungs of adult mice induced by Bisphenol A [BPA]
Study Design: An experimental study
Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi, in collaboration with the Animal House, National Institute of Health, Islamabad, from June to November 2016
Methodology: Thirty male and female BALB/c mice were divided into three groups, of 10 animals each. Group A animals served as control. Group B animals were given BPA at a dose of 50 mg/Kg body weight/day. Group C animals were given BPA and soyabean oil at doses of 50 mg/Kg body weight/day and 500 mg/day, respectively. All treatments were given once daily for a period of eight weeks. Animals were dissected 24 hours after receiving the last dose. Lung tissue specimen processing and H and E staining was carried out for routine histological study. Perivascular inflammation was morphometrically graded and statistically analysed using Chi-square test with p<0.05
Results: Grade 2 inflammation was recorded in two [20%] animals and grade 3 perivascular inflammation in 80% specimens in Group B; whereas 20% specimens of Group C had grade 2 inflammation and eight [80%] showed grade 1 inflammation. None of the control animals showed any inflammation. All groups were significantly different at p<0.001
Conclusion: BPA produced perivascular inflammation and con-commitant administration of soyabean oil diet protected against it in rodent
ABSTRACT
To evaluate the protective role of garlic extract on the histoloy of the uterine epithelium exposed to lead acetate in an animal model. Laboratory based randomized control trial. Department of Anatomy, Army Medical College in collaboration with National Institute of health from April to June 2013. Thirty female BALBc mice were selected. Ten animals were placed in each group. Group A being the control was given normal diet. Group B was given lead acetate at a dose of 30 mg/kg /day. Group C was given acetate 30 mg/kg/day and garlic extract 500 mg/kg/day through oral gavage tube for 60 days. Animals were sacrificed and dissected at the end of 60 days. Right uterine horn was processed, embedded and stained for histological study. Height of epithelium was measured. It was taken from apical to basal end of the cells. There was increase in height of the lining epithelium of uterus in group B, mean value 19.70 +/- 4.81 microm when compared to Group A, with mean value 13.25 +/- 2.37 microm. The height of the epithelium was relatively reduced in group C, with mean value 14.50 +/- 2.30 microm when compared with group B. In group C results were same as Group A. The p values were 0.001 when group A was compared to group B, 0.688 when group A was compared to group C and 0.005 when group B was compared to group C. The height of epithelium was markedly increased in lead acetate treated group which returned to normal when co treated with garlic extract. Hence garlic ameliorated the changes induced by lead
Subject(s)
Animals, Laboratory , Plant Extracts , Uterus , Epithelium , Mice, Inbred BALB C , Organometallic Compounds , Lead , AcetatesABSTRACT
To observe the effects of sodium cyclamate on islets of langerhans in rats pancreas. Laboratory based randomized control trial. Anatomy Department, Army Medical College Rawalpindi, in collaboration with National Institute of Health [NIH], Islamabad, from March to May 2014. Twenty male and twenty female Sprauge dawley rats weighing 175-205 gms were used in the experiment. Half male and half female rats were randomly divided in two groups [control group C and experimental group E, n=20 animals in each group]. Group C served as control group in which rats were given normal diet. Group E served as experimental group and was given sodium cyclamate 60mg/kg/day through oral gavage tube for two months. Animals were dissected. Pancreas was examined and weighed. Slides were made after processing the organ for histological study. Area of islets of langerhans was calculated by image j software. Results were analyzed on SPSS version 20. The mean weight of pancreas in control and experimental group was 0.75 gm [SD +/- 0.094] and 0.805 gm [SD +/- 0.068] respectively. It was significantly higher [p = 0.043] in experimental group. The area of islet of langerhans in control and experimental group was 15285.40 micro m[2] [IQR: 9881.08 - 23001.35] and 33213.50 micro m[2] [IQR: 21258.05-45879.18] respectively. There was an increase in area in experimental group [p = 0.014]. Sodium cyclamate affects the histomorphology of endocrine pancreas by increasing the area of islets of langerhans in treated group
ABSTRACT
To study the effect of Bicalutamide on testicular weight in adult rats. Study Design: Laboratory based randomized controlled trial. Anatomy Department, Armed Forces Postgraduate Medical Institute [AFPGMI], Rawalpindi, Pakistan in collaboration with National Veterinary Laboratories [NVL], Islamabad, Pakistan from April to May 2008. Forty adult male healthy Sprague Dawley rats with no physical deformity weighing 200-300 grams were randomly divided into two groups [A and B], each consisting of 20 animals each. Group A was taken as control group and was administered 5 ml of distilled water orally daily for 24 days while group B [experimental group] was given 5 ml of distilled water daily containing bicalutamide 10 mg/kg/day for 24 days. All the animals were sacrificed on the next day after the last dose. The testes were taken out and weighed. In addition to initial and final weights of the animals, weights of right and left testes were also taken, and their average was taken as a reading for that animal. Statistically significant difference was found in reduction of testicular weights in the testes of experimental group [1.112 +/- 0.018 g] when compared with the control group [1.169 +/- 0.011 g]. The results obtained showed that the mean testicular weight of the testes was significantly reduced in the experimental group thus confirming that bicalutamide reduces testicular weights in the Sprague - Dawley rats