Effect of wearing complete dentures on the candidal count in the oral cavity

Background: After the loss of natural teeth, dental prosthesis is of vital importance for the functional and esthetic restoration. Denture wearing is accepted as a pre disposing factor for oral candidiasis

Objective: To compare the candidal growth before and after the insertion of complete dentures

Materials and Methods: A total of forty patients wearing complete denture were included in this cross-sectional study. This study was conducted from 15[th] January, 2009 to 15[th] January, 2010 at department of Prosthodontics Lahore Medical and Dental College, Lahore. They were asked to rinse their mouth with 10ml of saline in fasting condition. The rinse was centrifuged and the deposit was sent to the Microbiology Laboratory for evaluation. The data was entered and analyzed in SPSS version 13

Results: At the time of insertion the candidal count zero in all patients and after one month of wearing denture 35% of the patients showed oral candidal growth. [P= 0.000]

Conclusion: The results of present study revealed that there is a significant quantitative increase in on the candidal count after complete denture wearing

Effect of gender, day and nighl use of complete denture on oral candidal growth

Background: Poor prosthetic hygiene is related with high level of oral candidal infection and many factors are being evaluated as its risk factors

Objective: The objective of present study was to determine whether gender and overnight denture wearing affects oral Candidal growth. Setting: Department of Prosthodontics, Lahore Medical and Dental College and Pathology Department, Microbiology Section of Lahore Medical and Dental College, Lahore

Patients and Methods: Forty edentulous patients including 20 male and 20 females, who fulfilled the inclusion criteria i.e. first time complete denture wearer between 50 to 65 years of age were included in this cross-sectional study. Exclusion Criteria: Patients with history of treatment with chemotherapy or radiotherapy in past six months, smokers and diabetic were excluded from the study. Oral rinse on same time a day technique was used for sample collection in which patient was provided 10 ml of sterile saline in a sterile disposable container and requested to rinse for 60 sec. It was sent to Microbiology section of Pathology Department, Lahore Medical and Dental College, Lahore. Qualitative and quantitative growth of Candida was studied. Microscopy, Gram stain and colony count was done

Results: Difference in candidal growth was significantly higher after one month of wearing complete denture in females. Results showed that overnight denture wearing caused a higher candidal growth

Conclusion: General population and specially, females should be given the awareness that their oral hygiene is important and dentures should be taken off at night, as they do with their eyeglasses. This will prevent denture stomatitis

Prevalence of MRSA in a peripheral hospital of Lahore

The rate of MRSA in increasing in hospitalized patients all over the world. The present study is done to find out the prevalence of MRSA in a peripheral hospital of Lahore over a period of one year from January to December 2009. Study was done by finding out percentage of MRSA among all the Staphylococcus aureus isolated from admitted patients of Ghurki Trust Teaching Hospital. Results show that 50% of all the S. aureus were MRSA

Candidal count in patients with complete dental prostheses

The aim of the analysis was to compare the candidal count at the time of insertion and after one month of wearing complete dentures. It was a quasi-experimental study which was carried out at Department of Prosthodontics, Lahore Medical and Dental College. Study group consisted of forty edentulous patients with no previous denture history. They obtained new complete dentures. Samples were taken using the oral rinse technique and cultured on sabouraud agar. Candidal colonies were detected and counted as colony forming units at the time of insertion and after one month of wearing complete dentures. To confirm presence of Candida gram staining and germ tube test were also carried out. Change in candidal count was significantly higher after one month of wearing complete dentures [p=0.0001]. Difference in candidal count was significantly higher in females than males. Candidal count was significant after wearing complete dentures and this should be considered as an important factor that can pre-dispose the patients to Candida induced denture stomatitis