Anti-hyperglycemic and antinociceptive activity of methanol leaf and stem extract of Nypa fruticans Wurmb

Nypa fruticans Wurmb. [Arecaceae] is a mangrove palm well-known for its traditional uses by the local practitioners against different ailments in southern regions of Bangladesh. However, the plant is yet to be scientifically studied. The present study was done to evaluate the anti-hyperglycemic and antinociceptive potential ofmethanolic extract of leaf and stem of Nypa fruticans Wurmb. [MENF]. The anti-hyperglycemic activity was tested on glucose loaded hyperglycemic mice whereas antinociceptive activity was evaluated using a model of acetic acid-induced writhing in mice. The crude MENF was found to show significant oral anti-hyperglycemic activity on glucose loaded mice at every dose. Maximum anti-hyperglycemic activity was observed at a dose of 500 mg MENF/kg body weight, which was more than what was obtained with a standard drug glibenclamide at a dose of 10 mg glibenclamide/kg body weight. Significant antinociceptive activity was also demonstrated by MENF in acetic acid-induced writhing mice model. The extract caused a maximum of 39.88% [p<0.00l] inhibition of writhing at the dose of 600 mg/kg body weight, which was better than the result obtained with a standard drug [200 mg aspirin/kg body weight, 49.34% inhibition]. These findings indicate that MENF has significant anti-hyperglycemic and antinociceptive activity and thus have great potential as a source of natural products

Antinociceptive and anti-hyperglycemic activity of methanol leaf extract of Cyperus scariosus

The objective of the present study was to investigate the antinociceptive and anti-hyperglycemic activity of methanolic leaf extract of Cyperus scariosus. Antinociceptive activity was determined using a model of acetic acid-induced gastric pain in mice and anti-hyperglycemic activity through glucose tolerance test using glucose loaded mice. In writhing assays induced by acetic acid, the methanolic leaf extract showed dose dependent significant pain inhibition compared to control. The maximum writhing inhibition [46.62%] was found at a dose of 200 mg/kg body weight which was less than that of the positive control, aspirin [56.74%], when used at the same dose. Anti-hyperglycemic activity of the extract was also found to be significant in mice loaded with glucose at doses of 200 and 400 mg/kg body weight. Maximum tolerance [42.86%] was showed at 400 mg extract/kg body weight, which compared favorably with that of glibenclamide at 10 mg/kg body weight [57.62%]. In summary, the methanol extract of C. scariosus leaves has had beneficial effects as a pain reliever and also in reducing the elevated blood glucose level of hyperglycemic mice