Cellular uptake characteristics of salvianolic acid B in myocardial cells and blood vessel endothelial cells / 中国药科大学学报

Aim: To determine the uptake characteristics of salvianolic acid B(Sal B) in myocardial cells and blood vessel endothelial cells. Method: The effects of various factors, such as time, temperature, drug concentration, pH of the medium, on the uptake of Sal B in myocardial cells and aorta endothelial cells were investigated. LC/MS was employed to determine the intracellular concentration of Sal B. Results: Uptake kinetics of Sal B in the myocardial cells and aorta endothelial cells fitted well to the logarithmic model at 37 ℃ and 4 ℃. The a-mount of uptake was in direct proportion to the extracellular concentration of Sal B in the experimental concentration range. Uptake of Sal B both in the myocardial cells and blood vessel endothelial cells would significantly increase while the medium pH decreased, and some water-soluble components extracted from danshen would also facilitate the uptake of Sal B both in the myocardial cells and blood vessel endothelial cells obviously. The energy metabolism inhibitors would significantly inhibit the uptake of Sal B in the myocardial cells and blood vessel endothelial cells. When lactic acid and fatty acid were added to the incubation solution, the uptake of Sal B both in the myocardial cells and aorta endothelial cells increased more than 20%. Conclusion: pH is the most important factor influencing the cellular uptake of Sal B, and the amount of uptake tends to increase in acidic medium. Results suggest that the uptake of Sal B would increase in the acidified internal environment induced by myocardial ischemia, thus exerting better cardiovascular activities.

Features and Implications of Fund-raising Activities Initiated by University Presidents during the Period of Republic of China——Take the Nankai University,Yanjing University and Southeast University for example / 中华医学教育探索杂志

During the period of Republic of China,the presidents of Nankai,Yanjing,and Southeast Universities raised funds actively from society under the condition of the shortage of the Government funding.The fund-raising was considered to be the most important activities in all of the work in three universities.They got enormous donations from the society and made a great success to the university.Today,university presidents still face the problem of lacking money.However,they attribute it mainly to the lacking financial supporting from the government.The fund-raising behaviors of the former presidents may give us some good inspirations for our modern university developments.