Effect of ischemic preconditioning on the apoptosis of hepatocytes and expression of regulating gene (bcl-2,Fas protiens) in rats / 中国普通外科杂志

Objective To explore whether hepatocellular ischemia reperfusion (IR) injury elicites the apoptosis of hepatocytes or ischemic preconditioning (IP) reduces the apoptosis (AP) of hepatocytes due to IR injury in the rat, and the effect of IP on AP regulating genes (bcl 2、Fas) protein expresssion. Methods Wistar rats were randomly divided into sham operation (SO) group, IR group and IP group. The latter two groups were futher divided into 3 subgroups respectively and subjected to 30 minutes of ischemia. IP was achieved by ischemia for 5 minutes, followed by reperfusion for 5 minutes before continuous block of the liver influe. The rats were killed after reperfusion for 1.5, 3 and 4.5 hours respectively, then liver tissues were sampled to examine the apoptosis and bcl 2 and Fas proteins. In SO group the rats were killed 3.5 hours after the operation and the liver tissues were sampled. Results Apotosis index of hepotocytes significantly in creased in IR groups compared with that in IP groups (P0.05). Expression of Fas protein was significantly increased in IR group and IP group than that in SO group (P0.05). Conclusions This study shows that: (1) IR injury may activate the apoptosis of hepatocytes by increasing Fas gene expression. (2) IP may decrease the apoptosis of hepatocytes by increasing bcl 2 gene expression.

Non-operative management for liver trauma (a report of 66 cases) / 中国普通外科杂志

Objective To investigate the clinical significance and indications of non operative management for liver trauma(LT). Methods The clinical date of 66 cases of LT treated by non operation from November 1987 to November 2000 were retrospectively analyzed. Results There were 38 cases (57.6%) in class I of LT, 18(27.3%) in class II, 10(15.2%) in class III in this series. 64 cases were cured (97.0%), including 2 cases combined with active bleeding were converted to operation and curred; 2 cases (3.0%) died of combining with severe brain damag. 4 cases(6.1%) complicated with liver abscess were cured by non operative treatment. Conclusions The non operative management is suitable for all cases of class I, II and partial cases of class III of LT. The observation of blood dynamics is most important, and B-type ultrasonography is also imporant, The operation would be done if the case is combined with massive active bleeding.