ABSTRACT
Three homoisoflavonoids, 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3 PO4 (58:42) as the mobile phase, and the detection wavelength was set at 296 nm (0-14 min) and 275 nm (14-22 min). The mean recoveries of three homoisoflavonoids were 99.41%-99.86% (RSD 0.82%-1.05%). The linear response ranges of. 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 microg (r = 0.999 9), 0.153-0.918 microg (r = 0.999 9), and 0.270-1.620 microg (r = 0.999 9), respectively. This method was certified to be accurate and reliable and can be used for quality control of O. japonicus.
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Isoflavones , Ophiopogon , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To determine the contents of twelve ginsenosides in the root of Panax ginseng by HPLC.</p><p><b>METHOD</b>The analysis is carried out at room temperature on a Luna NH2 column (4.6 mm x 150 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in a gradient elution. The flow-rate was 1.0 mL x min(-1), the detection wavelength was 203 nm.</p><p><b>RESULT</b>Twelve ginsenosides (Rh2, Rh1, Rg2, Rg3, Rg1, Rf, Re, Rd, Re, Rb2, Rb3, Rb1) were separated at baseline within 60 min with good linearity (r > or = 0.999 5). The recovery rates were 98.1%, 95.3%, 96.1%, 95.6%, 97.3%, 98.6%, 98.0%, 96.4%, 96.1%, 97.6%, 96.8%, 96.9% (RSD < or = 3.0%).</p><p><b>CONCLUSION</b>The method was simple,fast and could control the quality of P. ginseng effectively.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Ginsenosides , Panax , Chemistry , Plant Extracts , Plant Roots , Chemistry , Quality ControlABSTRACT
OBJECTIVE:To establish an RP-HPLC analysis of Carthamin in mice and to study its pharmacokinetics.METHODS:The serum concentration of Carthamin was determined by RP-HPLC.The blood concentration-time curve was established and the main pharmacokinetic parameters were computed.RESULTS:The linear range of Carthamin was 0.558～55.8 ?g?L-1(r=0.999 2),with the lowest limit of detection at 0.005 ?g?L-1Carthamin in vivo assumed two-compartment model and rapid absorption.CONCLUSION:The proposed method is simple,sensitive and reproducible,and it met the standard for pharmacokinetic study.
ABSTRACT
OBJECTIVE: To establish a HPLC method for the determination of the content of Demethylbellidifolin in different parts of Swertia davidi Franch. METHODS: The analysis was carried out on Hypersil C18 column (150mm?4.6mm,5 ?m) at room temperature with mobile phase consisted of CH3OH-0.5%H3PO4(56∶44) at a flow-rate of 1.0mL?min-1.The detection wavelength was set at 254 nm. RESULTS: The linear range of Demethylbellidifolin was 0.52～2.60?g (r=0.999 4) and the average recovery was 99.77%(RSD=0.95%).CONCLUSION: The method is simple, rapid, reproducible, and suitable for the determination of the content of Demethylbellidifolin in Swertia davidi Franch..
ABSTRACT
OBJECTIVE:To establish a HPLC method for the determination of quercitrin in Saxifrage.METHODS:The analysis was performed on C18 column(150mm?4.6mm,5?m),the mobile phase was MeOH-0.2%H3PO4 (45∶55)with a flow rate at 1.0ml/min and wavelength at 350nm under room temperature.RESULTS:There was a good linear relationship when the sample size of quercitrin was at a range of 0.40?g～2.00?g(r=0.9 996),the recovery was 95.33%with RSD at 2.80%.CONCLUSION:This method was simple,stable,fast,and reproducible and without the interference of impurity,which can be used for the content determination of quercitrin in Saxifrage.