ABSTRACT
OBJECTIVE: To observe the effects of astragalus polysaccharides(APS) combining with total ginsenoside (TG) on the proliferation of T cells in peripheral blood of healthy adults and on the immune function of mice after treatment with cyclophosphamide (CY) . METHODS: The proliferation rate of T cells in peripheral blood was detected by methyl thia-zolyl tetrazolium(MTT) colorimetric analysis; the weight of immune organs such as spleen and thymus etc. in mice were detected. RESULTS: The proliferation of T cells in human peripheral blood could be enhanced if the concentration of APS remained within 25-100?g/ml and that of TG remained within 5-20?g/ml; the proliferation rates were 102.3%-141.1% and 113.9%-163.1% , respectively; the figure stood at 262.3%-383.0% when APS and TG used in combination. The spleen indexes of APS group and the APS plus TG group increased significantly vs. the CY group(P
ABSTRACT
OBJECTIVE: To establish an HPLC method for the determination of fumaric acid in Pleurotm citrinopileatus. METHODS: HPLC was performed on Yilite C18 column(250 mm?4.6 mm,5 ?m) with methanol-0.01 mol?L-1 potassium dihydrogen phosphate solution(5 ∶ 95,pH adjusted to 2.5 using phosphoric acid) as mobile phase and detection wavelength of 216 nm. RESULTS: The linear range of fumaric acid was 0.16～1.6 ?g (r=0.999 9). The average recovery was 101.59% (RSD=1.28%,n=6). CONCLUSION: This method is accurate, simple and reproducible for the content determination of fumaric acid in P. citrinopileatus.