ABSTRACT
To study the in situ intestinal absorption of five oligosaccharides contained in Morinda officinalis How. (sucrose, kestose, nystose, 1F-Fructofuranosyinystose and Bajijiasu). The absorption of the five oligosaccharides in small intestine (duodenum, jejunum and ileum) and colon of rats and their contents were investigated by using in situ single-pass perfusion model and HPLC-ELSD. The effects of drug concentration, pH in perfusate and P-glycoprotein inhibitor on the intestinal absorption were investigated to define the intestinal absorption mechanism of the five oligosaccharides in rats. According to the results, all of the five oligosaccharides were absorbed in the whole intestine, and their absorption rates were affected by the pH of the perfusion solution, drug concentration and intestinal segments. Verapamil Hydrochloride could significantly increase the absorptive amount of sucrose and Bajijiasu, suggesting sucrose and Bajijiasu are P-gp's substrate. The five oligosaccharides are absorbed mainly through passive diffusion in the intestinal segments, without saturated absorption. They are absorbed well in all intestines and mainly in duodenum and jejunum.
Subject(s)
Animals , Male , Rats , Drugs, Chinese Herbal , Chemistry , Pharmacokinetics , Intestinal Absorption , Intestine, Small , Metabolism , Morinda , Chemistry , Oligosaccharides , Chemistry , Pharmacokinetics , Perfusion , Rats, Sprague-DawleyABSTRACT
Objective: To compare the UPLC and HPLC fingerprints of flavonoids in Citri Grandis Exocarpium (CGE). Methods: The HPLC and UPLC methods for analyzing the fingerprint of 17 samples were established respectively. Results: There were 21 mutual peaks selected from UPLC chromatography and 17 mutual peaks selected from HPLC chromatography. The similarity of 17 samples was over 0.9. Conclusion: Both the UPLC and HPLC methods could be used for the quality control of CGE. Compared to HPLC, the UPLC method is efficient, quickly, and sensitive.