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Chinese Pharmacological Bulletin ; (12): 1536-1541, 2021.
Article in Chinese | WPRIM | ID: wpr-1014510

ABSTRACT

Aim To investigate the effect of quercetin on the osteogenic ability of human dental pulp mesenchymal stromal cells (hDPSCs) in vitro and in vivo. Methods hDPSCs were obtained from the pulp tissues of premolar, and the characteristic surface antigens were identified by flow cytometry. Cell Counting Kit-8 (CCK-8) assay was used to test the cytotoxicity of quercetin. Alkaline phosphatase (A L P) and alizarin red staining were used to detect the osteogenic ability of cells in vitro. The expression of osteogenic genes was detected by qPCR. Four round calvarial bone defects with a diameter of 8 mm were created in 10 male New Zealand rabbits, and they were differentiated and randomized into four groups. Group A, hDPSCs cultured on Bio-Oss

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