ABSTRACT
Objective To investigate the Suidasia breeding in the flourmill and further observe its morphological characteris-tics.Methods The samples were collected from different habitats of a flourmill and then separated,and the chipping was kept. The mites were selected and counted from the chipping under an optical microscope. The mites were made slide samples and identified.Results The mites were identified as Suidasia(including Suidasia nesbitti and Suidasia medanensis).A total of 20 samples were collected in this study,of which 13 were positively detected,with the detection rate of 65%.There were 2 876 mites of Suidasia that were detected from 200 g sample.The average breeding density was 14.38/g.Conclusions It is common that Suidasia breeding in flour,which might cause human acariasis and allergic asthma.Therefore,the effective prevention mea-sures should be taken.
ABSTRACT
<p><b>BACKGROUND</b>Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.</p><p><b>METHODS</b>The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.</p><p><b>RESULTS</b>The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).</p><p><b>CONCLUSION</b>Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.</p>