ABSTRACT
Aim To study the effect of active components of isoflavones on PC12 cells damage induced with A?_(1-42). Methods The active components of isoflavones were extracted. Using PC12 cells damage induced by A?_(1-42) as the model,the protective effect of active components was determined with morphological examination and MTT and LDH assays. Results The morphological change of the damaged PC12 cells was improved, and the survival rate of the cells was significantly increased(P
ABSTRACT
AIM: To investigate the effect of recombinant human interleukin-10 (rhIL-10) on IL-6 and TNF-? levels in serum and liver of mice exposed to lipopolysaccharide (LPS). METHODS: rhIL-10 was prepared by using genetic engineering technology. Mice were intraperitoneally with 500 ?g of LPS, and then were treated intravenously with various dosages of rhIL-10. The levels of IL-6 and TNF-? in hepatic tissue and serum were determined by ELISA at 12 h, 24 h, 48 h and 72 h post rhIL-10 treatment. RESULTS: rhIL-10 markedly inhibited the increase in IL-6 and TNF-? levels in hepatic tissue and serum at 12 h after rhIL-10 treatment in LPS-challenged mice, and the inhibition effect was significant at 24-48 h after rhIL-10 treatment (P