Separation of isoflavones from Radix Pueraria by molecular imprinting technology / 中成药

AIM: To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer(MIP) and Scatchard analysis was carried out. METHODS: Pueraria MIP was synthesized using pueraria as the template,acrylamide as the functional monomer and ethylene glycol dimethaerylate(EDMA) as the crosslinker. RESULTS: The result showed that at least two classes of binding sites were formed in the imprinted polymer.Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION: The component of the products is analyzed by HPLC.The result shows that the final products contain pueraria and the other two kinds of isoflavone.