ABSTRACT
To evaluate the expression of cAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1α) in non-small cell lung cancer (NSCLC) and its correlation with clinicopathological features. Methods: PRKAR1α expressions in 79 NSCLC patients and matched adjacent non-carcinoma tissues were analyzed by using qRT-PCR and immunohistochemistry. Results: The negative rates of PRKAR1α protein in NSCLC, lung squamous cell carcinoma (SCL) and lung adenocarcinoma (ACL) were 58.2%, 77.8%, 32.4%, respectively. Compared to the matched adjacent non-carcinoma tissues, there were significant differences in levels of PRKAR1α mRNA and protein in ACL (P0.05). The expression of PRKAR1α protein was positively correlated with histological type, TNM stage, and lymph node metastasis (P0.05). Conclusion: Low expression of PRKAR1α in ACL might be involved in the pathogenesis, which might serve as a novel diagnostic candidate.
Subject(s)
Female , Humans , Male , Adenocarcinoma , Chemistry , Classification , Genetics , Adenocarcinoma of Lung , Biomarkers, Tumor , Carcinoma, Non-Small-Cell Lung , Chemistry , Genetics , Carcinoma, Squamous Cell , Chemistry , Genetics , Cyclic AMP-Dependent Protein Kinase RIalpha Subunit , Physiology , Gene Expression Profiling , Immunohistochemistry , Lung Neoplasms , Chemistry , Classification , Genetics , Lymphatic Metastasis , Genetics , Neoplasm Staging , RNA, MessengerABSTRACT
It was proposed that the items incurrent testing itemsdatabase should be classified , ranked , amal-gamated or disintegrated , the testing items and their names should be standardized , and the link between current testing itemsdatabase and new standards should be established according to the new standards by taking their problems into consideration, such as non-standard link, repetition of a same item, non-standard names of testing item and method, in order to improve the effectiveness of main testing methods, lay a foundation for the verifica-tion, and provide reference for the link between new and old items in other provinces.