ABSTRACT
Objective To investigate the clinical value of iTRAQ multiplex tandem mass spectrometry in the detection of the ex-pression of hepatocyte growth factor (HGF) in patients with invasive breast cancer .Methods A total of 35 patients with breast cancer and 30 healthy subjects were selected from January 2014 to October 2016 in this hospital ,the expression of serum HGF in breast cancer patients with different clinical stages and healthy subjects was analyzed by iTRAQ labeling ,mass spectrometry ,library searching and Scqffold software ,and the differential expression of HGF was verified by Western blot .Results A total of 237 pro-teins were identified in the serum samples of this study ,and 89 proteins with strict quantitative criteria ,17 differentially expressed proteins ,included HGF ,were screened for breast cancer patients and healthy controls .iTRAQ markers showed that the expression level of serum HGF in different clinical stage of breast cancer patients was significantly higher than that in healthy subjects (P<0 .05) .The results of Western blot showed that the relative expression level of serum HGF in breast cancer patients was significant-ly higher than that in healthy subjects(P<0 .05) .Conclusion iTRAQ multiplex tandem mass spectrometry is useful for the detection of breast cancer patients with high expression of HGF ,which is of great significance in guiding the clinical treatment of breast cancer .
ABSTRACT
Objective To investigate the clinical value of iTRAQ multiplex tandem mass spectrometry in the detection of the ex-pression of hepatocyte growth factor (HGF) in patients with invasive breast cancer .Methods A total of 35 patients with breast cancer and 30 healthy subjects were selected from January 2014 to October 2016 in this hospital ,the expression of serum HGF in breast cancer patients with different clinical stages and healthy subjects was analyzed by iTRAQ labeling ,mass spectrometry ,library searching and Scqffold software ,and the differential expression of HGF was verified by Western blot .Results A total of 237 pro-teins were identified in the serum samples of this study ,and 89 proteins with strict quantitative criteria ,17 differentially expressed proteins ,included HGF ,were screened for breast cancer patients and healthy controls .iTRAQ markers showed that the expression level of serum HGF in different clinical stage of breast cancer patients was significantly higher than that in healthy subjects (P<0 .05) .The results of Western blot showed that the relative expression level of serum HGF in breast cancer patients was significant-ly higher than that in healthy subjects(P<0 .05) .Conclusion iTRAQ multiplex tandem mass spectrometry is useful for the detection of breast cancer patients with high expression of HGF ,which is of great significance in guiding the clinical treatment of breast cancer .
ABSTRACT
Objective To explore the significance of quality control after clinical laboratory analysis .Methods A total of 450 pieces of unqualified testing reports were collected from the Department of Clinical Laboratory from January 2012 to June 2014 and reasons causing unqualified testing reports were analyzed .Results In all 450 pieces of unqualified testing reports ,testing results of 169 pieces were inconsistent with results of clinical diagnosis ,accounted for 37 .6% ;149 pieces with missing or indirect inspection i‐tems ,accounted for 33 .1% ;62 pieces did not indicate staff or department sending specimens ,accounted for 13 .8% ;results of 36 pieces reached the critical value but without re‐inspection or did not indicate the re‐inspection ,accounted for 8 .0% ;18 pieces did not clarify specimens with lipid turbidity or jaundice and so on ,accounted for 4 .0% ;16 pieces marked with wrong sample types ,accoun‐ted for 3 .6% .Conclusion It is necessary to conduct quality control after clinical laboratory analysis before delivering report ,stand‐ardize operating procedures ,check every report seriously ,make clear responsibility and improve awareness of responsibility ,in order to provide a qualified testing report for clinical practice .