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Objective:To investigate the effect of upregulated and downregulated PSMA7 on the cell cycle and Cyclin D1,CDK4,P16,Rb of RB pathway in A549 cells.Methods:Transfected upregulated pcDNA3.1-PSMA7 vecter and downregulated pGPU6/Hygro-PSMA7-265 vecter into A549 cells,and then tested the effect of PSMA7 on the cell cycle of A549 cells by flow cytometry,and detected the protein level of Cyclin D1,CDK4,P16,Rb by Western blot.Results:Compared with the control group,the cell cycle of the A549 cells did not change significantly,and the expression of Cyclin D1,CDK4 decreased but P16,Rb increased when PSMA7 was upregulated.Compared with the control group,the proportion of phase G0/G1,G2/M of the A549 cells decreased and phase S increased,and the expression of Cyclin D1,CDK4 increased but P16,Rb decreased when PSMA7 was downregulated.There was statistical significance for those results.Conclusion:PSMA7 could affect the expression of Cyclin D1,CDK4,P16,Rb protein level of RB pathway in A549 and promoted the A549 cells into phase S when it′s downregulated.
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Objective To perform the chromosome detection in 1 237 infertile patients for analyzing the karyotypes results and investigating the relationship between infertility and chromosome abnormalities .Methods The peripheral venous blood samples in 1 237 infertile patients in our hospital from March 2010 to December 2014 were collected ,performed the lymphocyte culture ,ob‐tained cells ,fixed under hypotonic condition ,prepared the section and observed them by microscope after G‐banding treatment .Re‐sults Among 1 237 patients ,111 cases abnormal karyotypes were detected with the total abnormal detection rate of 8 .9% ,in which ,57 cases were sex chromosomes abnormality ,54 cases were euchromosomes abnormality .Conclusion Chromosome abnor‐mality is one of the important causes leading to primary infertility .Infertility caused by chromosome abnormalities is irreversible ,so the chromosome cytogenetic examination is especially important in the diagnosis and treatment process of infertile patients .
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Objective To investigate the effects of health education on diet and behaviors in stegmonth. Methods Three hundred and forty three primiparas were divided into the experiment and control group.The 163 primiparas in the experiment group took the courses in the pregnant women’s school and 180 primiparas in the control group did not.The differences of diet and daily behaviors in stegmonth were compared between the two groups.Results There were statistically significant differences in the rate of diet,health behaviors,breast feeding between the two groups(P<0.01).Conclusion The health education for the pregnant woman can enhance their sense of self care,change their wrong behaviors,and improve the rate of breast feeding.
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To improve the teaching quality of neonatal diseases,we analyzed the diseases register of 760 neonates hospitalized in one year,two editions of textbook of pediatrics and the teaching outline,8 times of examination after the students had finished the exercitation.Accounted for 88.55%,673 cases of the neonates suffered from 15 diseases,which were included in the 17 diseases required by the teaching outline.Among the 669 cases of the neonates suffering from 10 common diseases,105 cases suffered from 5 diseases,which were not required by the teaching outline.As the second common disease,neonatal pneumonia was asked to be familiar with instead of mastering by the students.Accounted for 58.82%,10 diseases were required by the teaching outline without having them in the exams.Therefore it is important:1.to stand out the keystone on the clinic teaching of neonatal diseases such as neonatal jaundice,premature,and pneumonia which were required by the teaching outline as well as herpangina,vomit,humid lung-which were common diseases.2.to put neonatal vomit into the textbook and ask the students to master neonatal pneumonia.3.to perfect the setting of test paper:in the test paper neonatal diseases accounting for 20.00%,the diseases required by the teaching outline accounting for 85.00%.The proportion of important diseases to common diseases is 1.80∶1.
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We analyszed 108 examination papers that were taken when the students of 8 groups had finished theexercitation. The attainment was 73.6?4.4 points. Among the 400 selective questions, the difficult questions whichwere concentrated only on a few diseases accounted for 35.3%. There was mush difference between the proportion of theselective questions and that of the demands of the teaching program in different system of diseases. There was few or noselective question in the important diseases of the teaching program. It is suggested that the proposition of difficultquestions must include the important diseases in the teaching program. The diseases that are not commonly encountereddiseases may be deleted from the teaching program, but the commonly encountered must be put in the teaching program.It is necessary to reinforce the ability of students to analyse and resolve problems[
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Objective To investigate the relationship between newborn's feeding pattern and mother's knowledge-attitude-behavior in nutrition(K-A-B). Methods 246 pairs of mother and her newborn, 28?3 days old,were enrolled in the study,and questionnaire on the K-A-B was sent. Results The breaset-feeding rate of the newborn was 65.1%. 9.8% newborns were fed inappropriately. Only 65.5% mothers passed the examination of nutritional knowledge. However,88.5% mothers had positive attitude to receiving nutritional education. A lot of mothers have food preference. Nutritional lecture ,consultation in out patient department and by telephone were the nutritional education channels choiced by 78.5% mothers. Conclusion Breast-feeding rate of the newborns had been significantly reduced in comparison with that(85.1%) two years ago. Solid foods were given too early to the newborns. The major contributing factor is lack of knowledge in nutrition of mothers . Mothers should learn more about nutrition and improve their feeding skill through nutritional education.