ABSTRACT
Skin disorders are most common occurring diseases in places where humidity prevails throughout the year. Vicharchika and Eczema both having resemblance in set of their clinical signs. Rasapushpadi Malhara (RPM) is Rasa preparation described in Rasa tarangini which is indicated in some skin disorders like Phirang, Vrna, Vicharchaika etc. In present study two samples of Rasa Pushpa (RP) has been prepared by two methods i.e. Kupipakva method and Damru yantra method. Both the samples were further used to prepare two samples of Rasapushpadi Malhara using Siktha taila as base and ratio of Siktha & Til taila was taken in ratio of 1: 5 according to the season. The randomized control clinical trial was carried out in between two groups. Both groups showed significant results on different clinical parameters like Kandu, Daha, Srava, Rukshta, Pidika, Vaivaranyata, Pidika, Raji, but group B showed highly significant result in EASI Score, which indicates severity of eczema. Level of significance in Group B for EASI Score is, P<0.00001. Rasapushpadi Malahara is very potent in treatment of Vicharchika as it contains mineral and herbal drugs like Parada, Kasisa, Saindhava, Sphatika, Wax, Tila taila, all having good antibacterial and antifungal properties. So this formulation showed significant results only by external application.
ABSTRACT
Anticancer potential of Piper longum fruit against human cancer cell lines (DU-145 prostate, A549 lung, THP-1 leukemia, IGR-OVI-1 ovary and MCF-7 breast) as well as its in vitro and in vivo biochemical efficacy in AlCl3-induced hepatotoxicity were evaluated in the rats. Dried samples were extracted with several solvents using soxhlet apparatus. Flavonoid content in chloroform, benzene, ethyl alcohol and aqueous extracts of fruit was 19, 14, 12 and 11 μg quercetin equivalent/mg of sample, respectively. Hexane extracts exhibited 90-92% cytotoxicity against most of the test cell lines (A549, THP-1, IGR-OVI-1 and MCF-7), while benzene extract displayed 84-87% cytotoxicity against MCF-7, IGR-OV-1 and THP-1 cell lines. Among extracts, hexane, benzene and acetone extracts demonstrated considerable cytotoxicity (91-95%) against A549 (lung cancer) cell line in Sulforhodamine B dye (SRB) assay. Cell cycle analysis revealed that hexane, benzene and acetone extracts produced 41, 63 and 43% sub-G1 DNA fraction, demonstrating cell cycle inhibitory potential of these extracts against A549 cell line. Chloroform, ethyl alcohol and aqueous extracts displayed 71, 64 and 65% membrane protective activity, respectively in lipid peroxidation inhibition assay. P. longum fruit extracts also ameliorated AlCl3-induced hepatotoxicity, as indicated by alterations observed in serum enzymes ALP, SGOT and SGPT activity, as well as creatinine and bilirubin contents. In conclusion, study established the cytotoxic and hepatoprotective activity in P. longum extracts.