ABSTRACT
Cryptococcus spp. is a pathogenic fungus which is an increasingly important cause of infection, particularly in the immunocompromised hosts. Diagnosis of cryptococcosis in animals can be carried out by isolation of the fungus but this requires several days to detect and identify the organism. Detection of cryptococcal antigen by latex agglutination test and enzyme immunoassay in serum is a rapid and easy method for diagnosis of cryptococcosis. In the present study, a total of 142 blood samples were collected from apparently healthy (n=89) and diseased dogs (n=53) for diagnosis of cryptococcosis. Latex agglutination test and enzyme immunosorbent assay (EIA) were carried out for the detection of cryptococcal antigen in serum. Of the 142 serum samples tested, six samples tested positive by Latex agglutination test while one sample tested positive by EIA. The sample which was positive by EIA was also positive by Latex agglutination test. The serum samples of dogs that tested positive for cryptococcal antigen were obtained from dogs suffering from symptoms like bloody faeces and vomit, emesis, chronic ear infection and discharge. Based on our findings, we conclude that the latex agglutination test in combination with the enzyme immunoassay can be used for the diagnosis of Cryptococcosis in dogs
ABSTRACT
Background: Slums are not new phenomenon. They are characterized by insanitary condition, crime, drug abuse etc. The male youth living in the slums, are prone to various health impacting conditions including substance use like alcohol consumption, smoking, tobacco chewing, drugs etc.Methods: Cross-sectional study was conducted in the four selected urban slums of Amritsar city. From each slum 250 male youth aged 15-24 years were included in the study, so total 1000 respondents were interviewed and required information was filled in pre-designed semi-structured proforma. Data was compiled, analysed and valid conclusions were drawn.Results: The overall prevalence of substance use among the respondents was 25.9% and as the age increases the prevalence of substance use also increases. Among substance users, tobacco (86.8%) was the most common substance consumed both in smoking form (52.1%) and smokeless form (34.7%), followed by the alcohol (72.2%) and then drugs (12%). Majority (62.1%) of respondents had initiated the substance use between 15-19 years of age. Factors like illiteracy, migration, joint family, married status and increased mental stress were significantly associated with substance use, however no association was found with socio economic status of the respondents.Conclusions: Government should take robust steps to improve the awareness and education status of the youth living in the slums. As the younger ages are most vulnerable ones, health education, IEC and BCC activities regarding the harmful effects of substance use along with life skills should be incorporated into the school curriculum.