ABSTRACT
The objective of this study was to evaluate practice of universal infection control protocols among third and final year BDS students at different dental colleges. Comparative study. This study was conducted in five dental colleges and hospitals which are affiliated with University of Karachi during the year 2011-2012. The undergraduates [third and final professional BDS students were selected with the age range from 18-22 years and without gender discrimination in the year 2011-2012. A self applied, confidential 14 close-ended type questionnaire consisting of various aspects of infection control practice was distributed to these undergraduates at the end of second semester after the lecture with permission and consent of head of institution. The collected data was analyzed by using SPPS 16.0. Among 180 students, 90[50%] were final year students and 90[50%] were third year students. Out of them 70 final year and 72 third year students participated. Thus a total of 142 [77.77%] students completed questionnaire with a response rate of 79%. The final year students were found to have more knowledge and had practiced more infection control procedures than third year students. Compliance with recommended guidelines for cross infection control varies among final year and third year students. Efforts are needed to improve attitudes to implement information and motivate students in the correct and routine use of infection control measures before they commence their clinical rotations
ABSTRACT
The objective of this study was the detection and separation of S. aureus from blood cultures of patients undergoing oral surgical procedures. Antibiotic sensitivity pattern and biofilm formation of S. aureus were also performed. Total 250 patients undergoing oral surgical procedures were selected for bacteriological examination. 5ml of Blood sample was collected in blood culture bottles containing tryptone soya broth. Blood sample was incubated at 37[degree sign] C for 7 days and after incubation subculturing was done on appropriate Media. The plates were then incubated at 37[degree sign]C aerobically for 24 hours, after which isolated colonies were obtained. S.aureus was identified by Gram staining, colony morphology, pigment production, catalase, coagulase and often biochemical tests. Antibiotic susceptibility was performed by disc diffusion technique on isosensitivity agar. Strains of S.aureus were used for biofilm formation by simple tubemethod. With the help of spectrophotometer at 570 nm optical density was measured. S.aureus [ATTC2523] was analyzed for biofilm production. Bacterial isolates in descending order were S.aureus 56%, E.coli 25%, Pseudomonas spp. 13%, S.typhi 4%and Shigella spp2%. S.aureus was resistant to different antibiotics. Biofilm production of S.aureus was detected in 16.17%of the S.aureus and mostly in association with antibiotic resistant bacteria. S.aureus was the predominant group of bacteria isolated from blood cultures of dental patients. Increased antibiotic resistance of S.aureus may be due to biofilm production resulting in persistent dental infections