ABSTRACT
After removal of both the lateral eyes of external gill stage tadpoles of the toad Bufo melanostictus, the pineal organ gets transformed into a median eye. This type of transformation occurs in tadpoles of both control and vitamin A treated groups. However, vitamin A increases the likelihood of homeotic regeneration (57% in the control group and 71% in the vitamin A treated group). Histological studies showed that the newly transformed median eye developed from the pineal organ. The pineal eye so developed possessed all components of a normal eye such as a retina, sensory cells and lens.
Subject(s)
Animals , Bufonidae/growth & development , Genes, Homeobox , Larva/drug effects , Regeneration/drug effects , Vitamin A/pharmacologyABSTRACT
The effect of vitamin A has been studied on lens regeneration in young (7 days old) as well as adult mice. A longitudinal slit was made under local anesthesia in the cornea over the lens. The lens was extracted intact through the incision. Intraperitonial injection of vitamin A (0.05 ml of 30 IU/ml in young and 0.05 ml of 50 IU/ml in adult) was given to the operated animals. Vitamin A was found to induce lens regeneration in not only young but also in adult mice. Regenerated lenses were similar in shape, size, transparency and histological features to normal intact lenses.