Physicochemical properties and skin penetration in vitro of total alkaloids of Sophora flavescens nanoemulsion / 中国中药杂志

The research aimed at investigating the physicochemical properties, stability and skin penetration in vitro of total alkaloids of Sophora flavescens nanoemulsion. Prepare total alkaloids of S. flavescens nanoemulsion and detect the determination of matrine and oxymatrine in the nanoemulsion using HPLC method. Transmission electron microscopy and laser particle size analyzer were utilized to detect the shape and size of the nanoemulsion respectively. And also the stability of nanoemulsion was studied under the conditions of low temperature (4 degrees C), normal temperature (25 degrees C) and high temperature (60 degrees C). Franz diffusion cell was used to research the transdermal absorption of nanoemulsion in vitro. The results found that the nanoemulsion we prepared presented appearance of rounded, uniform; its average diameter was (15.55 +/- 2.24) nm, and particle size distribution value was 0. 161; the appearance, diameter and percentage determination of total alkaloids of S. flavescens had no variations after 15 d under 4, 25, 60 degrees C respectively. The steady-state permeation rate was 4.564 1 microg x cm(-2) x h(-1), 24 h cumulative amount of penetration was 110.7 microg x cm(-2), which was 1.86 fold of 24 h cumulative amount of aqueous solution (59.41 microg x cm(-2)). All the results demonstrated total alkaloids of S. flavescens nanoemulsion had good permeability, and could provide a new preparation for its clinical application.

Preparation technology for phospholipids complex of total flavonoids from leaves of Diospyros kaki / 中草药

Objective: To study the influence factors in the preparation process for phospholipids complex of total flavonoids from the leaves of Diospyros kaki (PC-TF-LDK) and to optimize the conditions for the formation. Methods: The solvent method was used to prepare the PC-TF-LDK, and the optimized conditions were obtained by means of single factor test and orthogonal design test with the complex ratio as evaluation criterion. The formation of the complex was analyzed by the X-ray diffraction and differential scanning calorimetry. Results: The formation of the PC-TF-LDK was greatly influenced by the ratio of solvents, TF-LDK, and soybean phospholipid. The optimized preparation conditions for the PC-TF-LDK were obtained as follows: the solvent was anhydrous ethanol, the ratio of TF-LDK to soybean phospholipid was 1:2, the mass of reaction concentration was 30 mg/mL, temperature was 30°C, and reaction time was 1 h. Under this condition, 96.95% complex ratio was achieved. Conclusion: The preparation technology for PC-TF-LDK is reliable, stable, and available for industrial production.