ABSTRACT
Objective To observe the effect of electrical stimulation combined with bladder function training on autonomic bladder pa-tients after S2-4spinal cord injury or cauda equina and pelvic nerve injury.Methods From January,2016 to August,2017,76 patients with au-tonomic bladder after spinal cord injury were divided into control group(n=29)and observation groups(n=47)in real world study.The con-trol group received routine bladder function training,and the observation group received bladder muscle stimulation therapy in addition.The micturition diary and urodynamic indexes were assessed before and three months after treatment.Results After treatment,the frequency of urinary incontinence and the number of micturition in 24 hours,and residual urine volume significantly reduced(t>3.251,P<0.01),the urine output every time significantly increased (t>5.000, P<0.001), the maximum bladder capacity and urine flow rate increased (t>1.922, P<0.05),and the detrusor pressure in the end of filling period significantly reduced(t>8.302,P<0.001).All the indexes above were better in the observation group than in the control group(t>1.919,P<0.05). Conclusion Electrical muscular stimulation combined with the bladder func-tional training could improve the urination function in the patients with autonomic bladder after spinal cord injury.
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Objective To observe the effect of Kinesio taping on subluxation of shoulder in hemiplegic patients after stroke. Methods From October, 2015 to October, 2016, 40 hemiplegic patients after stroke were randomly divided into control group (n=20) and test group (n=20). They were administered with routine rehabilitation, and the test group accepted Kinesios taping and the control group accepted pla-cebo taping. The gap between acromion and caput humeri in X-ray film was measured before and after four weeks of treatment respectively, while they were assessed with Simplified Fugl-Meyer Assessment (FMA) of upper limb. Results The gap between acromion and caput hu-meri was less in the test group than in the control group after treatment (Z=-2.004, P<0.05), while the score of FMA was more (t=2.492, P<0.05). Conclusion Kenesio taping may prevent subluxation of shoulder in hemiplegic patients after stroke, and promote the recovery of mo-tor function of upper limbs.
ABSTRACT
Objective To observe the effect of Kinesio taping on subluxation of shoulder in hemiplegic patients after stroke. Methods From October, 2015 to October, 2016, 40 hemiplegic patients after stroke were randomly divided into control group (n=20) and test group (n=20). They were administered with routine rehabilitation, and the test group accepted Kinesios taping and the control group accepted pla-cebo taping. The gap between acromion and caput humeri in X-ray film was measured before and after four weeks of treatment respectively, while they were assessed with Simplified Fugl-Meyer Assessment (FMA) of upper limb. Results The gap between acromion and caput hu-meri was less in the test group than in the control group after treatment (Z=-2.004, P<0.05), while the score of FMA was more (t=2.492, P<0.05). Conclusion Kenesio taping may prevent subluxation of shoulder in hemiplegic patients after stroke, and promote the recovery of mo-tor function of upper limbs.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of a microencapsule scaffold capable of sustained release of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) in promoting the osteogenic differentiation of rat periosteum-derived stem cells (PDSCs) in vitro.</p><p><b>METHODS</b>PDSCs from 4-week-old SD rats, after identification of the surface markers using flow cytometry, were induced to differentiate into osteoblast, chondroblast, and adipocyte lineages. The differentiated cells were verified by staining with Alizarin red, toluidine blue, alcian blue, oil red O and by immunofluorescence assay. FGF-2/PELA/BMP-2, FGF-2/PELA, PELA/BMP-2 and PELA microcapsules were prepared, examined for surface morphologies using scanning electron microscopy (SEM), and tested for controlled release of FGF-2 and BMP-2 using ELISA. The third passage of PDSCs were cultured in the presence of the aqueous extracts of one of the 4 materials, and alkaline phosphatase (AKP) activity in the culture media was detected at 7 and 14 days of culture; the expression levels of osteogenesis-related genes were quantified with quantitative real-time PCR (qRT-PCR). The osteogenic differentiation ability of the PDSCs cultured with the extracts was compared.</p><p><b>RESULTS</b>The PDSCs, which expressed mesenchymal stem cell surface markers, were shown to have osteogenic, chondrogenic and adipogenic differentiation potentials. The cells cultured with the extract of FGF-2/PELA/BMP-2 microcapsules showed the highest AKP activity at 7 and 14 days of culture, and their expression levels of OCN and RunX-2 mRNA were the highest among the 4 groups; RunX-2 expression reached its peak level on day 14, and OCN mRNA expression level increased progressively as the culture time extended.</p><p><b>CONCLUSION</b>FGF-2/PELA/BMP-2 biomimetic controlled release microcapsules preserve the cytokine activities and are capable of promoting the osteogenic differentiation of rat PDSCs.</p>