ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-tumor metastatic constituents from Ardisia Crenata.</p><p><b>METHOD</b>Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.</p><p><b>RESULT</b>Nine compounds(1-9) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: 5-hydroxymethyl-2-furalclehyde(1), ethyl-beta-D-fructopyranoside(2), syringic acid(3), n-butyl-beta-D-fructofuranoside(4), n-butyl-alpha-D-fructofuranoside(5), methyl-alpha-D-fructofuranoside(6), (+)-bergenin(7), ardisiacrispins B(8), asperuloside acid(9). The isolated compounds(1-9) showed positive anti-tumor metastatic activities, and compounds 1, 5, and 8 showed significant anti-tumor metastatic activities. At the concentration of 0.8 mg x L(-1), compound 5 revealed the value of metastatic inhibition ratio on MDA-MB-231 was 93.8%.</p><p><b>CONCLUSION</b>Compounds 2-6 and 9 were isolated from this plant for the first time. compounds 1, 5 and 8 showed significant anti-tumor metastatic activities.</p>
Subject(s)
Humans , Antineoplastic Agents , Pharmacology , Therapeutic Uses , Ardisia , Chemistry , Cell Line, Tumor , Cell Proliferation , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Neoplasm Metastasis , Drug TherapyABSTRACT
<p><b>OBJECTIVE</b>To study the anti-tumor metastatic constituents from Lindera glauca.</p><p><b>METHOD</b>Constituent isolation and purification was carried by repeated column chromatography (silica gel, Toyopearl HW-40 and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.</p><p><b>RESULT</b>Ten compounds (1 - 10) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: Laurotetanine (1), N-methyllaurotetanine (2), reticuline (3), pallidine (4), N-trans-feruloyltyramine (5), N-cis-feruloyltyramine (6), atheroline (7), norisosocorydine (8), [9,9,9-(2) H3]-(1S*, 3S*, 4S*, 8S*)-p-menthane-3,8-diol (9), [9,9,9-(2) H3 ]-(1S*, 3R*, 4S*, 8S*)-p-Menthane-3,8-diol (10). Compounds 1, 2, 4, 5, 7 and 9 showed positive anti-tumor metastatic activities,and compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.</p><p><b>CONCLUSION</b>Compound 3 was isolated from this plant for the first time. Compounds 9 and 10 were isolated from Lindera genus for the first time. Compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.</p>
Subject(s)
Humans , Alkaloids , Chemistry , Antineoplastic Agents, Phytogenic , Chemistry , Aporphines , Chemistry , Benzylisoquinolines , Chemistry , Cell Line, Tumor , Chromatography, High Pressure Liquid , Methods , Lindera , Chemistry , Monoterpenes , Chemistry , Neoplasm Metastasis , Plant Extracts , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the immunosuppressive constituents from Tetraena mongolica.</p><p><b>METHOD</b>Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The MTT assay was applied to evaluate the isolated compounds on the inhibition effect of lymphocyte transformation.</p><p><b>RESULT</b>Six triterpenes were isolated and their structures were identified as follows: 3beta-hydroxy-11alpha, 12alpha:13beta,28-diepoxyoleanane(1), 3beta-(3, 4-dihydroxycinnamoyl)-erythrodi-ol(2), olean-28-al-3beta-yl-caffeate(3), erythrodiol (4), 12-oleanaen-3beta-caffeate(5), 3-O-(E) -coumaroylerythrodiol(6). Compound 24 exhibited the inhibition effects on lymphocyte transformation.</p><p><b>CONCLUSION</b>Compounds 1-6 were isolated from this plant for the first time, and compound 1 was a new nature product. Compound 2-4 showed significant immunosuppressive activity.</p>
Subject(s)
Animals , Male , Mice , Cells, Cultured , Immunosuppressive Agents , Chemistry , Pharmacology , Lymphocyte Activation , Magnetic Resonance Spectroscopy , Mice, Inbred BALB C , Triterpenes , Chemistry , Pharmacology , Zygophyllaceae , ChemistryABSTRACT
Objective To study the chemical constituents from Onychium japonicum.Methods Che-mical constituents were isolated by repeated column chromatography and preparative HPLC,and their structures were elucidated on the basis of spectroscopic method.Results Ten compounds were identifed as:4,3',4'-trihydroxy-2,6-dimethoxychalcone(1),chrysoeriol(2),luteolin(3),butin(4),protocatechuic acid(5),3,4-dihydroxy-acetophenone(6),caffeic acid(7),vanillic acid(8),2,4-dihydroxybenzaldehyde(9),syringic acid(10),and ?-sitosterol(11).Conclusion Compound 1 is a new product named japonicone D,compounds 2-10 are isolated from the plants of Onychium Kaulf for the first time.