Effect of compressive stress on the expression of DNAX-activating protein 12 and tartrate-resistant acid phosphatase in mouse monocyte RAW264.7 / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the expression of DNAX-activating protein 12 (DAP12) and tartrate-resistant acid phosphatase (TRAP) in mouse monocyte RAW264.7 subjected to compressive stress.</p><p><b>METHODS</b>Mouse monocyte RAW264.7 was subjected to four-point bending system. The expression of DAP12 and TRAP mRNA were examined by reverse transcription-polymerase chain reaction (RT-PCR) and the expression of DAP12 protein by Western Blotting after 0, 3, 6 and 12h's compressive stress.</p><p><b>RESULTS</b>After RAW264.7 cells were cultured by osteoclast cell culture fluid, the amount of the cells increased, and the volume enlarged, and the number of nuclei per osteoclast increased in vitro. The expression of DAP12 and TRAP mRNA and DAP12 protein of RAW264.7 cells subjected to compressive stress increased along with the time (P < 0.05).</p><p><b>CONCLUSION</b>The mouse monocyte RAW264.7 can differentiate into osteoclast in vitro, and high expression of DAP12 exists in the process of osteoclast differentiation.</p>

A study on expression of platelet derived growth factor a in periodontal tissue during orthodontic tooth movement with rotating pulsed magnetic field / 中国医师杂志

Objective To investigate the effects of rotating pulsed magnetic field on platelet derived growth factor a (PDGF-A) expression in periodontal tissue during tooth movement. Methods 30 white rabbits were random divided into 6 groups with 5 rabbits each group,including groups of 1,3,5,7,14 and 21 days. Under anesthesia condition by 2% pentobarbital sodium,the stainless coil springs were fixed between the first maxillary molar and the incisor producing the force of 80g. The experimental group was treated by rotating pulsed magnetic field and the force, the control group was only treated by t he force. The expression of PDGF-A was half-quantitatively investigated through immunohistochemical analysis. Results The expression of PDGF-AA in the experimental group enhanced apparently compared with that in the control group. There were significant differences among the 5,7, and 14day groups (5. 28 ± 0. 14 vs 2. 03 ±0. 18,7.63±0.27 vs 2. 84 ±0. 12,3.52 ±0. 16 vs 1.65 ±0.03;8.10±0.13 vs 4. 30 ±0. 21,13. 27 ±0. 31 vs 6.47 ± 0.15,5.66 ± 0.22 vs 3. 15 ± 0. 27, P ＜ 0. 05). The expression of PDGF-AA of 7 day group was higher than that of other groups. Conclusion Rotating pulsed magnetic field promotes the expression of PDGF-A in the periodontal tissue and alveolar bone remodeling.