ABSTRACT
OBJECTIVE To establish the ultra-high liquid chromatography （UPLC） characteristic spectrum of Uncariae Ramulus Cum Uncis from different producing areas， to conduct chemical pattern recognition analysis， and to identify the medicinal materials of their different origins and counterfeit products. METHODS UPLC method was adopted to establish the characteristic spectra of 43 batches of Uncariae Ramulus Cum Uncis from different origins； cluster analysis combined with principal component analysis were used to analyze their quality； Uncariae Ramulus Cum Uncis from different origins and counterfeit products were identified. RESULTS UPLC specific spectrum of Uncariae Ramulus Cum Uncis was established， and 13 common peaks were calibrated； peak 2 was identified as catechin， peak 3 as chlorogenic acid， peak 4 as cryptochlorogenic acid， peak 7 as isochlorogenic acid B， peak 8 as isodehydroguotenine， peak 9 as isooguotenine， peak 10 as dehydroguotenine， peak 11 as isochlorogenic acid C， peak 12 as goutenine， and peak 13 as camptothecin. Through cluster analysis， the medicinal materials of 43 batches of Uncariae Ramulus Cum Uncis could be divided into 5 categories according to their different origins. Further principal component analysis revealed that the principal component comprehensive scores of Uncariae Ramulus Cum Uncis produced in Jiangxi and Hunan were relatively high， ranging from 0.264 to 2.904. The specific chromatogram could effectively distinguish among the different origins and their counterfeit products of Uncariae Ramulus Cum Uncis. CONCLUSIONS The established UPLC specific chromatogram can be used for quality control of Uncariae Ramulus Cum Uncis， and the study found that the quality of Uncariae Ramulus Cum Uncis from Jiangxi and Hunan provinces is relatively good.
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Objective To evaluate the effect of schistosomiasis control in Yizhou City,Guangxi Zhuang Autonomous Re?gion,from 2011 to 2014,so as to provide the evidence for formulating the further prevention and control strategy. Methods The schistosomiasis surveillance data were collected and analyzed in Yizhou City from 2011 to 2014. Results From 2011 to 2014,770.38 hm2 was surveyed for the Oncomelania hupensis snails,and two snail infested sites were found,while no infected snails were found. Totally 3 524 residents were tested by ELISA for Schistosoma japonicum infection,and 38 cases were posi?tive. The positive rate of 2013 was significantly higher than those of other three years( χ2 = 15.08,P < 0.05). Totally 432 rats and 28 dogs were dissected and 1 697 cattle were examined by the stool test,but no positive cases were found. Conclusions The indicators of schistosomiasis surveillance are basically stable in Yizhou City.
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A new mathematical equation characterizing the compression of pharmaceutical materials is presented. This equation presumed that the rate of change of the compressible volume of powder with respect to the pressure is proportional to the compressible volume. The new model provided a good fit to several model substances employing non-linear regression techniques. The validity of the model had been verified with experimental results of various pharmaceutical powders according to the Akaikes informatics criterion (AIC) and the sum of squared deviations (SS). The parameter of the new model might reflect quantitatively the fundamental compression behaviors of the powders. It had demonstrated that the proposed model could well predict the compaction characteristics of solid particles like the Kawakita model.
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Aim: To prepare novel aqueous polymer dispersions with high flexibility for sustained-release coating and investigate their properties. Methods: The aqueous polymer dispersions were synthesized by the emulsion polymerization. The physico-chemical properties and film-forming potential of the dispersions were investigated while the mechanical properties of the formed film and the drug release behavior when atenolol pellets were coated with the aqueous polymer dispersions were evaluated. Results: The prepared aqueous polymer dispersions (methyl methacrylate/ethyl acrylate, 1:2) were found to have proper physico-chemical properties, excellent film-forming capability and satisfying mechanical properties. It could form free film with high flexibility and low viscosity in low temperature even in absence of the plasticizer. Sustained release of atenolol pellets was achieved when the pellets were coated the polymer dispersions. 4-h and 8-h cumulative releases were more than 50% and 80%, respectively. There was no significant difference in release between pellets prior to and post compression of the coated pellets. Conclusion: The resulting aqueous polymer dispersions could be used as sustained-release coating material with high flexibility suitable for tableting.
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BACKGROUND: Agiotensin Ⅰ -converting enzyme (ACE) inhibitory peptides which are separated from red tilapia skin collagen should be researched further.OBJECTIVE: To obtain a peptide of high ACE inhibitory activity through enzymic hydrolysates of red tilapia skin collagen.DESIGN: Enzymic hydrolysates were done with orthogonal experimental method; decompression peptide was separated with ultrafiltration, gel chromatography and hypertensive liquid chromatography.SETTING: Laboratory of Aquatic Products, Chinese Marine University.MATERIALS: The red tilapia weighing (500+50)g was donated by Branch Factory of Jimo Redian Factory. ACE was isolated from hog lung.METHODS: The experiment was carried out in the Laboratory of Aquatic Products, Chinese Marine University from May Bergman.①The collagen from red tilapia skin was extracted using the method described by Grossman and Bergman.The collagen extraction was hydrolyzed with compound enzymes in the order of bromelain and Alcalase 2.4 L.The red tilapia collagen hydrolysates (RTCH) were subsequently boiled to inactivate the enzyme. The resultant RTCH was fractionated through three different UF membrane bioreactor system having a range of molecular weight cut-offs (MWCO) of Mr 6 000, 4 000 and 1 000. Three portions were obtained: RTCH-I (M, 6000-4000), RTCH- Ⅱ (Mr 4000-1000)and RTCH-Ⅲ(Mr＜1000).②The ACE inhibitory 50%of ACE activity was defined as the IC50 value.③RTCH-Ⅲ(1.5 Ml) with the highest activity among RTCHs was loaded onto a Sephadex G-25 column (1.6×100 cm), and the absorbance of theeluent was monitored at 220 nm. Four samples of primary hydrolysates, RTCH- Ⅰ, RTCH- Ⅱ and RTCHⅢ were separated to collect active fractions which were pooled and lyophilized, immediately. The lyophilized fraction was separated with HPLC ODS-C18 analysis column to obtain component of high activity. Meanwhile, the same method was used to measure ACE inhibitory rate.④Each sample was hydrolyzed with 6 mol/L hydrochloric acid containing 1g/L thioglycolic acid at 110 ℃ for 24 hours in vacuum. The amino acid compositions of the resultant hydrolysates were determined on an amino acid auto analyzer, and molecular weight was measured with HPLC technique.MAIN OUTCOME MEASURES：①ACE inhibitory activity of fractionated RTCHS;②Purification of ACE inhibitory peptide;③Amino acid analysis of ACE inhibitory peptides.RESULTS:①ACE inhibitory activityof fractionated RTCHs:IC50values of RTCH-Ⅰ,RTCH-Ⅱand RTCH-Ⅲ were 3.30,2.23 and 0.31 g/L,and inhibitory of RTCH-Ⅲ was the highest.②Purification of ACE inhibitory peptide: The IC50 value of the four peak were 3.5, 2.12, 1.56 and 0.65 g/L, respectively. Results in Figures 2, 3, 4 and 5 showed that the high active fractions were: 6K4, 4K2, 1K2 and ACF2, and the inhibitory ratio were 11.1%, 89.9%, 65.0% and 49.7%, respectively.Among of these fractions,the 4K2 exhibited the highest inhibitory rate.③Amino acid analysis of ACE inhibitory peptides: Separated peptide products had more aromatic and hydrophobic amino acids.CONCLUSTON: Enzymic hydrolysates of red tilapia skin collagen have high ACE inhibitory peptide which is separated with ultrafiltration, gel chromatography and hypertensive liquid chromatography.