ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of artemisiae annuae CQ-189 (AACQ-189) on proliferation of hNSC and HELF in vitro, and the main organ toxicity and the median lethal dose (LD50) of kunming mouse in vivo. The purpose is to approach that the toxicity and side effects of AACQ-189.</p><p><b>METHOD</b>Using techniques of the colorimetric 5-diphenyl tetrazolium bromide (MTT) to detect the effects of AACQ-189 on proliferation of hNSC, and to detect the number of HELF survival by using techniques of trypan blue exclusion. To detect LD50 by tail vein injection in kunming mouse and using histomorphology method to observe the mouse main organ damage by AACQ-189.</p><p><b>RESULT</b>AACQ-189 has low poisonous function on hNSC and HELF that our experimental concentration (3.125-12.5 mg x L(-1)) has already achieve an effective dose to inhibit the proliferation of Leukemia cells obviously. LD50 concentration of kunming mouse is 550 mg x kg(-1). Moreover, AACQ-189 has little effect to main organs at higher concentration.</p><p><b>CONCLUSION</b>AACQ-189 has low poisonous function, which is a natural anti-tumor drug and has a promising prospect for potential application. However we should do more research on its mechanism.</p>
Subject(s)
Animals , Female , Humans , Male , Mice , Artemisia , Chemistry , Cell Line, Tumor , Cell Proliferation , Cells, Cultured , Drug-Related Side Effects and Adverse Reactions , Fibroblasts , Cell Biology , Lung , Cell Biology , Plant Extracts , Pharmacology , Stem CellsABSTRACT
Objective: To investigate the effects of artemisinic acid on proliferation of K562 cells and its mechanism in vitro.Methods: MTT assay was used to detect the effects of artemisinic acid on proliferation of K562 cells.The flow cytometry was used to analyze the distribution of cell cycle.Morphological changes of cells were observed by electron microscopy.Results: The proliferation of K562 cells was inhibited by intervention of artemisinic acid significantly after 24 hours and 48 hours,and the effects were in concentration-dependent manner.The cell number in G0/G1 increased and apoptosis was induced after the treatment with artemisinic acid for 48 hours,the morphological characteristic of apoptosis was observed by electron microscopy.Conclusion: Artemisinic acid can inhibit the proliferation of K562 cells,which may be-due to induction of apoptosis and prohibit on of tumor cells from entering cell cycle.