ABSTRACT
To observe the feasibility and safety of general anesthesia without muscle relaxant tracheal intubation combined with right stellate ganglion block (SGB) on patients undergoing oropharyngeal surgery.60 patients undergoing selective oropharyngeal surgery were randomly and equally divided into 2 groups:named in non muscle relaxation group and combination group.MAP,HR,SpO2 and PETCO2 were recorded before administration (T0),immediately before tracheal intubation(T1),and immediately after tracheal intubation(T2),and immediately after skin incision (T3).The VAS score at 4,8,12,24 h after surgery were also recorded.The difference of the satisfactory intubation conditions was not statistically significant.MAP and HR were increased at T2 and T3 as compared with non muscle relaxation group.Compared with combination group,HR increased at T2 and T3 in group A.The VAS of patients in combination group was lower than non muscle relaxation group (P ＜ 0.05).Stellate ganglion block on patients undergoing oropharyngeal surgery in general anesthesia without muscle relaxant might provide not only satisfactory intubation conditions but also provoke earlier recovery and improve the quality of postoperartive analgesia.
ABSTRACT
Objective To investigate the effect of isoflurane pretreatment on hypoxia/reoxygenation (H/R)-induced syndecan-1 shedding from human umbilical vein endothelial cells (HUVECs) . Methods HUVECs were cultured in EMB-2 medium and randomly divided into 3 groups ( n = 32 each) : control group (group C), H/R group and isoflurane pretreatment group (group I). H/R was produced by 4 h exposure of HUVECs to hypoxia followed by 2 h reoxygenation in H/R and I groups. HUVECs were exposed to the mixture of 5% CO2 and 95% O2 for 30 min and then cultured in normal culture atmosphere (21% O2) in group C. In group I, HUVECs were expased to 1.73% isoflurane and incubated for 30 min before H/R. The syndecan-1 expression, concentrations of shed syndecan-1 in the medium, and cell permeability and viability were measured at the end of reoxygenation. Results Compared with group C, the shed syndecan-1 concentration in the medium and cell permeability were significantly increased, while the syndecan-1 expression and cell viability decreased in H/R and Ⅰ groups ( P ＜ 0.01) . Compared with group H/R, the shed syndecan-1 concentration in the medium and cell permeability were significantly decreased, while the syndecan-1 expression and cell viability increased in group Ⅰ (P ＜ 0.01) . Conclusion Isoflurane pretreatment can protect HUVECs against H/R injury through inhibiting the syndecan-1 shedding.