ABSTRACT
Atrazine [ATZ] is one of the most commonly used herbicides that adversely affect the reproductive system in rats. The aim of the study was to evaluate the impact of vitamin E [Vit E] on subchronic exposure to ATZ in testicular tissue. Fifty adult male albino rats were divided into five groups: the negative control group; the positive control group, which received 1 ml of corn oil/day, orally; the Vit E group, which received Vit E at 100 mg/kg/day, orally; the ATZ group, which received ATZ at 300 mg/kg/day, orally; and the ATZ+Vit E group, which received both ATZ and Vit E at the previously mentioned doses. Treatments were given for 6 days/week for 45 days. At the end of the experiment, blood samples were taken to measure serum testosterone level. Semen analysis and estimation of oxidative stress markers catalase, superoxide dismutase, and tissue malondialdehyde were carried out. The testes were processed for light and electron microscopic examination. An immunohistochemical study was performed for detection of Bcl2. ATZ caused a decrease in serum testosterone level and in superoxide dismutase and catalase enzyme activities, whereas malondialdehyde content increased. There was also a decrease in sperm count, viability, and motility in comparison with the control groups. Light microscopic examination of seminiferous tubules revealed degeneration of the germinal epithelium. The lumen contained sloughed cells and homogenous acidophilic material. Ultrastructurally, there was separation of the germinal epithelial cells with small dense nuclei and phagocytic remnants. Sertoli and Leydig cells were also affected. Bcl-2 immunolocalization revealed weak reaction in the cytoplasm of the germinal epithelial cells and Leydig cells. Concomitant use of Vit E caused partial improvement. ATZ induced detrimental effects in the testicular tissue that were attenuated by concomitant administration of vitamin E