ABSTRACT
OBJECTIVE: To study the chemical constituents of the whole herb of Liparis japonica. METHODS: The compounds were isolated by column chromatography on silica gel, ODS and Sephadex LH-20 columns. Their structures were identified by analysis of physical and spectroscopic data and confirmed by comparison of their spectral data with those in the literature. RESULTS: Eighteen compounds were isolated and identified respectively as acortatarin A (1), 3β-hydroxy-5α, 8α-epidioxyergosta-6, 22-diene (2), 13-epicupressic acid (3), 3β-hydroxyolean-12-en-28-oic acid (4), betulinic acid (5), gigantol (6), (2S)-3-O-octadeca-9Z, 12Z, 15Z-trienoylglyceryl-O-β-D-galactopyranoside (7), linolenic acid(8), hydroxydihydrobovolide(5-hydroxyl-3, 4-dimethy-5-penty-12(5H)-furanone)(9), trans-p-hydroxycinnamic acid (10), (+)-syringaresinol (11), β-sitosterol (12), hircinol (13), nudol (14), moscatin (15), batatasin III (16), linoleic acid (17), and p-hydroxybenzaldehyde (18). CONCLUSION: We obtained structurally unique spiroalkaloid with morphine structure from the genus Liparis. Compounds 2-11 are isolated for the first time from this genus for the first time.