ABSTRACT
AIM@#To identify the structure of the acid-catalyzed product of strictosamide and explore the reaction mechanism.@*METHODS@#The acid-catalyzed reaction process of strictosamide was monitored by HPLC, and a macroporous resin was used to purify the reaction solution. The structure of the product was confirmed by MS, NMR, and ROESY spectra.@*RESULTS@#The acid-catalyzed transformation yield from strictosamide to vincoside lactam was 52%.@*CONCLUSION@#The reaction mechanism of the transformation from strictosamide to vincoside lactam may be related to the stability of the three-dimensional configuration of the compound. These results offer a new way to obtain vincoside lactam from the widely distributed indole alkaloid strictosamide by acid-catalysis.
Subject(s)
Acids , Chemistry , Catalysis , Lactams , Chemistry , Molecular Structure , Vinca Alkaloids , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To determine the content of polyphenol contained samples, in order to provide basis for comprehensive quality evaluation on Echinacea extracts.</p><p><b>METHOD</b>LC-MS was used for qualitative analysis on relevant compounds of the samples of Echinacea extracts. Optimized USP methods, RP-HPLC and QAMS (quantitative analysis of multi-components by single-marker) were used to determine the content of polyphenol. Meanwhile, UV method was adopted for comparative analysis on content according to international standard IS014502-1-2005.</p><p><b>RESULT</b>The six batches of samples could not meet the export standard for polyphenol content by HPLC. UV showed four batches in line with the standard and two batches in inconformity.</p><p><b>CONCLUSION</b>UV method is generally adoptedfor the content determination of domestic Echinacea extracts, but shows results that are significantly different from that by HPLC method. it is suggested to use HPLC method to standardize quality of extracts and raise market access standards.</p>