ABSTRACT
OBJECTIVE: To summarize the experience of bariatric and metabolic surgery in a single center from 2009 to2017 and explore the methods of the specialty construction of bariatric and metabolic surgery. METHODS: The clinical data of 1139 cases of bariatric and metabolic procedures performed from October 2009 to December 2017 in Department of Bariatric and Metabolic Surgery,the First Affiliated Hospital of Nanjing Medical University were analyzed retrospectively. RESULTS: The case numbers increased annually after the foundation of bariatric and metabolic surgery with the annual growth rate of 40.1%. Bleeding and leakage were the main postoperative complications. Among them,there was one case of postoperative death,6 cases of re-operations within the 30-day after discharge,and 21 cases of 30-day postoperative readmission. The learning curve analysis indicated that learning time was 23 cases for laparoscopic gastric bypass and 25 cases for sleeve gastrectomy. The rates of 1-year excessive weight loss were 83.0% for gastric bypass,72.4% for sleeve gastrectomy,and 82.3% for sleeve gastrectomy plus jejunal bypass. The rates of 1,3 and 5-year follow-up were around 95.0%,67.0% and 52.0%. CONCLUSION: The foundation of bariatric and metabolic specialty is the key reason for the massive increase of surgical cases. The learning curve of laparoscopic bariatric and metabolic surgery can be shortened though systemic specialty training. The collaboration of multi-disciplinary team and the perioperative process management are the key points for the construction of an excellent center.
ABSTRACT
Objective: To construct the recombinant lentiviral vector expressing specific shRNA of p120 catenin gene, and to identify the RNA interference efficiency by infecting PANC-1 cells. Methods: Three pairs of shRNA targeting p120ctn mRNA were designed, synthesized and ligated into enzyme-digested and linearized pGCSIL-GFP lentiviral vectors. The recombinant lentiviral vectors were co-transfected with the pGC-FU-p120ctn-3FLAG containing p120ctn gene into 293T cells after identification by PCR and sequencing analysis. Western blotting analysis was applied to select the most effective shRNA. Recombined lentivirus was packaged and the concentration of the virus titer was measured. Real-time PCR and Western blotting analysis were used to identify the interference efficiency after infection of the PANC-1 cells by recombined lentivirus. Results: PCR and DNA sequencing analysis confirmed that p120ctn-shRNA-LV was successfully constructed and the concentration of the virus titer was 3×109TU/ml. Real-time PCR showed that expression of p120ctn mRNA was decreased by 82.6% in PANC-1 cells infected with the recombined lentivirus (P<0.05). Western blotting analysis showed that the expression of p120ctn protein was also greatly deceased after infection. Conclusion: We have successfully constructed lentiviral vector expressing shRNA of p120 catenin gene, and this lays a foundation for studying the role of p120ctn in invasion and metastasis of pancreatic carcinoma.