Application of FRET in fluorescence detection of FQ-PCR / 中国医疗器械杂志

The theory of fluorescence resonance energy transfer (FRET) and methods of fluorescence detection in fluorescent-quantitative polymerase chain reaction (FQ-PCR) are introduced in this article. Applications of FRET in fluorescence detection of PCR are emphatically discussed, and FRET research progress and future trends are pointed out too.