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<p><b>OBJECTIVE</b>To explore the effect of microneedle combined with Lauromacrogol on skin capillary network.</p><p><b>METHODS</b>24 male Leghone (1.5-2.0 kg in weight) were randomly divided into three groups as group A (microneedle combined with Lauromacrogol), B (microneedle combined with physiological saline) , and C(control). The cockscombs were treated. The specimens were taken on the 7th, 14th, 21th , and 28th day postoperatively. HE staining, immunohistochemical staining and special staining were performed for study of the number of capillary and collagen I/III , as well as elastic fibers.</p><p><b>RESULTS</b>The color of cockscombs in group A became lightening after treatment. The number of capillary decreased as showing by HE staining. The collagen I and III in group B was significantly different from that in group A and C (P < 0.05). Special staining showed proliferation of elastic fibers in group B.</p><p><b>CONCLUSIONS</b>It indicates that microneedle combined with Lauromacrogol could effectively reduce the capillary in cockscomb without any tissue fibrosis. Microneedle can stimulate the proliferation of elastic fiber, so as to improve the skin ageing process.</p>
Subject(s)
Animals , Male , Capillaries , Chickens , Comb and Wattles , Needles , Polyethylene Glycols , Pharmacology , Punctures , Methods , Random Allocation , Skin AgingABSTRACT
10.3969/j.issn.2095-4344.2013.26.010
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Objective To investigate the effects of different concentrations of lauromacrogol intravenous injection on the thrombosis and vascular walls through the animal experiment.Methods Thirty ear marginal veins of rabbits whose weight ranged from 2.5 kg to 3.0 kg and whose sex was not limited were divided randomly into six groups:1.0 ％ lauromacrogol group,0.9 ％ lauromacrogol group,0.8 ％ lauromacrogol group,0.7 ％ lauromacrogol group,0.6 ％ lauromacrogol group and normal saline group.The local veins and their side tissue were cut off for samples for HE staining and VEGF expression detected by immunohistochemistry at the first day,third day,fifth day and seventh day after injection.Results Visual observation and HE staining showed that 1.0 ％ lauromacrogol group,0.9 ％ lauromacrogol group,0.8 ％ lauromacrogol group and 0.7 ％ lauromacrogol group had thrombosis in veins after injection,0.6 ％ lauromacrogol group and normal saline group did not have thrombosis in veins after injection.The score of VEGF expression showed that 1.0 ％ lauromacrogol group,0.9 ％ lauromacrogol group,0.8 ％ lauromacrogol group and 0.7 ％ lauromacrogol group did not have statistically significant differences between groups and different time point (P＞ 0.05) and there were statistically significant differences between 0.6 ％ lauromacrogol group and other four groups (P＜0.05) in each time point.Conclusions From these animal experiments lauromacrogol shows the effect of vascular injury and thrombosis,eventually leading to the injected vein disappeared in 0.7 ％ lauromacrogol group.
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Objective To investigate the behaviors character of men who have sex with men(MSM) in the local place,learn about the prevail situation of STI and HIV/AIDS among MSM group,and provide evidences for the health promotions on AIDS and STI among MSM.Methods Based on a snowball model,survey on MSM were conducted with an anonymous model.3～5 ml vein blood of the MSM were also collected at the same time.Results The awareness rate of HIV/AIDS information among 452 MSM was high.98.45% can give the right answer to question of whether would be infected with HIV if inject blood or blood product which have been infected with HIV.Total understand rate was 92.86%.The rate of condom use in their last anal sex with male was 64.43%.Rate of never using condom and condom using in every anal sex with male in the late 6 months was 10.95% and 34.83% respectively.The 3 services of HIV that most been welcomed by them were IEC(67.92%),condom distribution(62.61%) and lube distribution(57.30%).1.99% has a positive result on anti-HIV testing. 8.85% have a positive result on syphilis testing.Conclusions High dangerous behaviors are prevalent among MSM in Nanning and propaganda on HIV/AIDS information is not sufficient.Multi-types approaches should be adopted to prevent the epidemics of HIV/AIDS among MSM.
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BACKGROUND:Study shows that margarita liquid has effect on promoting histiocyte regeneration and removing scars.OBJECTIVE:To observe the effects of margarita liquid on the proliferation of fibroblasts in human skin scar tissues.DESIGN,TIME AND SETTING:A grouping contrast observational experiment was performed in the Experiment Centre of Guangxi Medical University from September to December in 2008.MATERIALS:Scar tissue samples were obtained from patients in the Department of Plastic Surgery,Guangxi Medical University.Margarita liquid was the digest of margarita purchased from Beihai Gofar Marine Biological Industry Co.,Ltd and rich in multi-amino acids,polypeptides,vitamin,mineral matters and natural enzymes.METHODS:Human skin scar cell line was established by removing non-fibroblasts through repeated primary culture and serial subcultivation of scar fibroblasts with reference to Veelken method. The 3-5 generation human skin scar fibroblasts on exponential phase of growth were made into single cell suspension by trypsin digestion which was then inoculated on plastic 96-well cell culture plate,with the density of 0.5×10~4/well as well as 100 μL cell suspension and 100 μL DMEM medium in each well. After culture for 24 hours,primary medium was discarded. The grouping of the experiment:200 μL mediums with 125.00,62.50,31.30,15.60,7.80,3.90,1.95,0.98 mg/L margarita liquid were used respectively in margarita liquid group;200 μL pure medium was added into each well in control group.MAIN OUTCOME MEASURES:MTT and TUNEL assay were used to examine the proliferation and the apoptosis of fibroblastsrespectively.RESULTS:The 50% inhibiting concentration (IC_(50)) of margarita liquid on fibroblasts was 15 mg/L. Margarita liquid at any other concentration but 0.98 mg/L was effective in inhibiting fibroblast growth in a dose-dependent way,i.e. the higher margarita liquidconcentration,the higher inhibition ratio on fibroblast growth. Fibroblasts cultured with 15 mg/L (IC_(50)50) margarita liquid had got reduced volume,lessened cytoplasm,decreased density,increased apoptosis rate and buffy colour. Fibroblasts in control group were large,rich in cytoplasm and compact. Apoptotic index was higher in the margarita liquid group than in the blank control group CONCLUSION:Margarita liquid could inhibit the proliferation of skin scar fibroblasts cultured in vitro and induce the apoptosis of them.
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Objective To evaluate the preventive and therapeutic effects of artemisinin and artesunate on hypertrophic scar in rabbit ears.Methods Full-thickness wounds to cartilage were created in New Zealand white rabbit cars to establish animal models of hypertrophic scar.Cream was prepared with artemisinin or artesunate.A total of 96 hypertrophic scars were divided into 4 groups to receive the treatment with artemisinin cream.artesunate cream.cream vehicle(vehicle control)or no treatment(blank control)28 days after wounds were created.After 28-day treatment,animals were scarified,scars were incised and examined with HE-staining and VG-staining.Hypertrophy index.numerical density of fibroblasts and area density of collagen fibers were calculated.Results Compared with vehicle and blank controls,the scars were softer and flatter,the volume of fibroblasts decreased,and collagen fibers appeared to be more regulated and sparse in artemisiIlin or artesunate cream-treated groups.The Hypertrophy index.numerical density of fibroblasts.area density of collagen fibers were(1.452±0.27),(3638.245±463.0)cells/mm2,(32.29±6.9)%in artemisinin cream-treated group,respectively,(1.445±0.24),(3585.016±638.9)cells/mm2,(34.74±8.27)%in artesunate cream-treated group,respectively.All the three parameters were significantly reduced in artemisinin and artesunate groups than in blank and vehicle control groups(all P＜0.0 1).but no significant difference was found between artemisinin and artesunate groups (P＞0.05).Conclusion Artemisinin and artestmate cream has a reliable efficacy in the prevention and treatment of hypertrophic scar in animal models.