Cytotoxic and proliferous effects of Nitraria tangutorum flavone on human A-704 strain cells / 中国药理学通报

Aim To observe the cytotoxic and proliferous effects of Nitraria tangutorum flavone(NTF)on human A-704 strain cells.Methods In vitro cultured human A-704 strain cells,the cytotoxic and proliferous effects of NTF on human A-704 strain cells were determined with methods of trypan-blue and clone formation,and the microelement in cell was detected with X-electron microscope scanning.Results ① The trypan-blue detection showed that the living cells in the NTF group(0.1,0.5 and 5 mg?L-1)and the 5-FU group were decreased by 33.8%~57.3%,35.8%~80.1%,60.8%~99% and 40.3%~59.6% at 6~18 hour after treatment,which significantly differed from those of the control group(P