Papers published in PLoS One by Chinese authors engaged in scientific research / 中华医学图书情报杂志

Objective To analyze the characteristics of contributions and the reasons why Chinese authors engaged in scientific research favor to contribute their papers to PLoS One by comparing the papers published in PLoS One and its series by authors from China and other countries.Methods Papers published in PLoS One by Chinese au-thors engaged in scientific research were retrieved from Web of Science by setting the retrieval parameters and ana-lyzed.Results The number of papers published in PLoS One series by authors from China increased rapidly.The number of papers published in PLoS One was significantly larger than that published in other PLoS One series. Conclusion The scientific research scale is unceasingly expanded.Why the Chinese authors engaged in scientific research favor to contribute their papers to PLoS One are due to its attractive power and the guidance of scientific and technological evaluation management in China.

Detection of GATA5 gene methylation in plasma and stool of colorectal cancer and the clinical ;diagnosis / 中国癌症杂志

Background and purpose:Colorectal cancer (CRC) is a malignancy which is the third incidence and the fourth mortality in the worldwide. The main reason for the development of CRC is that the changes of genetic and epigenetic causes the tumor suppressor gene methylation silencing. This study aimed to investigate the plasma and stool GATA5 gene promoter methylation was detected in clinical diagnosis of CRC. Methods: To collect the paired plasma and stool specimens of 34 cases of healthy and 43 cases of patients with CRC. Methylation speciifc PCR (MSP) was respectively detected the GATA5 gene methylation levels of GATA5 gene in plasma and stool. And then separately analyzed their correlations with clinical and pathological parameters in gastric carcinoma. Results: The result of MSP showed that GATA5 gene promoter methylation rates in plasma and stool of CRC patients were 60.74%, 76.60%, respectively, were higher than those of healthy persons (6.47%, 32.35%). And the differences were statistically signiifcant (P=0.006 7, P=0.000 2, respectively). GATA5 gene methylation rates in plasma of CRC patients were closely related to clinical stage (P=0.000 5) and lymph node metastasis(P=0.020), while GATA5 gene methylation rates in stool of CRC patients had no signiifcant with clinical and pathological parameters. Conclusion:Detection of faecal GATA5 gene methylation level and supplemented plasma GATA5 gene methylation level can become a simple, non-invasive, sensitive and speciifc method for clinical diagnosis of CRC.