ABSTRACT
Objective:To investigate the expressions of CC chemokine receptor 3 (CCR3) and CC chemokine 5 (CCL5) in serum of patients with non-small cell lung cancer (NSCLC) and their predictive value for recurrence and metastasis after surgery.Methods:The clinical data of 90 NSCLC patients (NSCLC group) who underwent surgery in Rugao People's Hospital from January 2017 to December 2020 were retrospectively analyzed. At the same time, 95 healthy people who underwent physical examination during the same period were selected as the healthy control group. The levels of serum CCR3 and CCL5 in the two groups were detected by enzyme-linked immunosorbent assay. After a 2-year postoperative follow-up, the NSCLC patients were recorded for recurrence and metastasis after surgery. Multivariate logistic regression analysis was used to identify the independent factors affecting patients' recurrence and metastasis after surgery. Receiver operating characteristic (ROC) curve was used to evaluate the predictive efficacy of CCR3 and CCL5 levels for patients' recurrence and metastasis after surgery.Results:The levels of CCR3 and CCL5 in NSCLC group were higher than those in the healthy control group [(290±36) pg/ml vs. (183±23) pg/ml; (15.7±3.1) pg/ml vs. (6.2±1.2) pg/ml], and the differences were statistically significant ( t values were 24.02 and 27.24, both P < 0.001). The incidence of recurrence and metastasis after surgery in NSCLC group was 34.44% (31/90). The levels of CCR3 and CCL5 in patients with recurrence and metastasis were higher than those in patients without recurrence and metastasis [(315±39) pg/ml vs. (276±35) pg/ml; (17.5±3.4) pg/ml vs. (14.7±2.9) pg/ml], and the differences were statistically significant ( t values were 4.87 and 4.04, both P < 0.001). The result of univariate analysis showed that the proportions of patients with stage Ⅲ A, poorly differentiation and lymph node metastasis in patients with recurrence and metastasis were higher than those in patients without recurrence and metastasis (all P < 0.05). Multivariate logistic regression analysis showed that TNM stage ( OR = 2.587, 95% CI 1.509-4.434), CCR3 level ( OR = 2.215, 95% CI 1.354-3.623) and CCL5 level ( OR = 2.343, 95% CI 1.193-4.598) were independent factors affecting patients' recurrence and metastasis after surgery (all P < 0.05). ROC curve analysis showed that the areas under the curve (AUC) of CCR3, CCL5 and combination of the two in predicting NSCLC patients' recurrence and metastasis after surgery were 0.710, 0.684 and 0.854. Conclusions:The abnormal high expressions of CCR3 and CCL5 in the serum of NSCLC patients are independent risk factors for recurrence and metastasis after surgery. The combined detection of CCR3 and CCL5 can effectively predict the recurrence and metastasis of patients after surgery.
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Objective To explore the relationship between the expression of human telomerase RNA component (TERC) gene , human papilloma virus (HPV) infection and mutation of chromosome 3 number with cervical lesions .Methods 81 women received the treatment in the Gynecology Department of the Second Affiliated Hospital of Kunming Medical University from June 2008 to February 2009 ,including the healthy group(normal pathological examination ,20 cases) ,CIN1 group(28 cases) ,CIN2 group(12 ca‐ses) ,CIN3 group(9 cases) and cervical cancer group(12 cases) .The TERC gene expression in uterine epithelial exfoliated cells was detected by using the fluorescence in situ hybridization (FISH) method ,meanwhile the HPV infection was detected by using the real time fluorescence quantitative polymerase chain reaction (FQPCR) technology .The correlation between cervical cancer with TERC gene and HPV was analyzed .At the same time the number of chromosome 3 mutations in 81 cases was recorded .Results In the cervical lesion detection ,the detection positive rate had no statistical difference between the TERC gene detection and HPV detec ‐tion (P> 0 .05) ,their positive rates in the CIN 1 ,CIN2 ,CIN3 and cervical cancer groups were significantly higher than that in the healthy group (P 0 .05) , while between the CIN3 group and the cervical cancer group had statistical significance (P< 0 .05) ,the higher the malignant degree , the higher the positive rate .The abnormal mutation rate of chromosome 3 number was 0% in the healthy group and the CIN1 group ,16 .7% in the CIN2 group ,66 .7% in the CIN3 group and 100 .0% in the cervical cancer group ,the positive rate in the CIN3 group and the cervical cancer group was significantly higher than that in the healthy group ,CIN1 group and CIN2 group ,the differ‐ences were statistically significant (P< 0 .05) .Conclusion The TERC abnormal gene expression ,high risk HPV infection and mutation of chromosome 3 number could play an important synergistic effect during the process of occurrence and progression of cervical cancer .