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Objective To evaluate the application value of glycerol blood chocolate soup in the bacteria preservation of Haemophilus influenzae.Methods Haemophilus influenzae strains ATCC10211,ATCC49247 and ATCC49766 were saved into glycerol blood chocolate soup,then it was stored in refrigerator at below -35℃.The preserved strains from the refrigerator at 1 month,3 months,6 months,1 year,2 years were taken out,and dissolved it at 35℃ for 5min,and then immediately transferred it to chocolate agar plate,placed it in the 35℃,CO2 environment overnight.Observed the survival of strains,colony morphology and whether the demand factor test had change.Saved the standard strains ATCC49247 and ATCC49766 2 years for the paper dispersion method drug sensitive test.Results After 2 years of observation,3 strains of Haemophilus influenzae strains grew well in the survival rate of 100%,the colony was colorless,transparent and flat moist,it could be observed gram small negative bacilli by Gram staining, blood agar satellite test was positive,no hemolysis,Columbia satellite test was negative.All traits were the characteristics of Haemophilus influenzae.The drug sensitivity characteristics of ATCC49247,ATCC49766 standard strains were still in line with the requirements of CLSI.Conclusion Glycerol blood chocolate soup is suit for Haemophilus influenzae bacteria preservation.
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OBJECTIVE:To discuss effects of Bifid triple viable capsules combined with Dahuang chishao decoction on endo-toxemia and intestinal mucosal barrier function of patients with hepatocirrhosis. METHODS:90 patients with hepatocirrhosis were randomly divided into traditional Chinese and western medicine group and western medicine group with 45 cases in each group. Both groups were given symptomatic treatment,such as liver protection and diuresis,lowering portal pressure and nutrition sup-port. Based on this,western medicine group was given Bifid triple viable capsules 0.42 g,tid,po;traditional Chinese and western medicine group was additionally given Dahuang chishao decoction orally,one dose a day,on the basis of western medicine group. Both 2 groups were treated for 6 weeks. The changes of liver function index,serum endotoxin(ET),procalcitonin(PCT)and di-amine oxidase (DAO) levels of patients were observed and recorded in 2 groups before and after treatment. RESULTS:After 6 weeks treatment,serum ALT,AST and TBIL,ET,PCT and DAO levels of patients in 2 groups declined significantly,compared with before treatment,with statistical significance(P<0.05 or P<0.01);the decrease of traditional Chinese and western medicine group was more significant than western medicine group,with statistical significance(P<0.05). CONCLUSIONS:Bifid triple via-ble capsules combined with Dahuang chishao decoction can reduce ALT,AST and TBIL levels in patients with hepatocirrhosis,pro-tect liver function;reduce serum ET,PCT and DAO levels,release endotoxemia,and protect and remedy intestinal mucosal barri-er function.
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Objective To evaluate the quality and application value of improved sheep blood chocolate medium.Methods The ATCC 10211 of Haemophilus influenzae was inoculated into the modified medium and unmodified medium,the average growth index(GI)of Haemophilus influenzae in two kinds of culture medium was compared.Based on 352 selected qualified sputum specimens for detection of Haemophilus influenzae,the positive isolation rate of medium was compared between the two groups.Results GI value of the traditional blood chocolate culture medium was (3.69 ±0.58),which was significantly lower than (15.08 ±1.34)of the improved sheep blood chocolate culture medium,the difference was significant (t =25.31,P <0.01 ).352 sputum specimens in the improved sheep blood chocolate culture medium,Haemophilus influenzae detected in 41 cases,the positive rate was 11.65%.And 352 sputum specimens in traditional sheep blood chocolate culture,Haemophilus influenzae detected in 18 cases,the positive rate was 5.54%.There was significant differenceof Haemophilus influenzae in separation based on 2 kinds of chocolate culture medium (χ2 =21.04,P <0.05).Conclusion Haemophilus influenzae significantly improved sheep blood colony in chocolate culture medium,grows well,easily to be identified,which helps to detect sputum specimens of Haemophilus influenzae.